Scholars@Duke publication: Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV.

Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV.

Publication , Journal Article

Brown, EP; Weiner, JA; Lin, S; Natarajan, H; Normandin, E; Barouch, DH; Alter, G; Sarzotti-Kelsoe, M; Ackerman, ME

Published in: J Immunol Methods

April 2018

The Fc Array is a multiplexed assay that assesses the Fc domain characteristics of antigen-specific antibodies with the potential to evaluate up to 500 antigen specificities simultaneously. Antigen-specific antibodies are captured on antigen-conjugated beads and their functional capacity is probed via an array of Fc-binding proteins including antibody subclassing reagents, Fcγ receptors, complement proteins, and lectins. Here we present the results of the optimization and formal qualification of the Fc Array, performed in compliance with Good Clinical Laboratory Practice (GCLP) guidelines. Assay conditions were optimized for performance and reproducibility, and the final version of the assay was then evaluated for specificity, accuracy, precision, limits of detection and quantitation, linearity, range and robustness.

Duke Scholars

Author Marcella Sarzotti-Kelsoe Integrative Immunobiology

Published In

J Immunol Methods

DOI

10.1016/j.jim.2018.01.013

EISSN

1872-7905

Publication Date

April 2018

Volume

455

Start / End Page

24 / 33

Location

Netherlands

Related Subject Headings

Simian immunodeficiency virus
Simian Immunodeficiency Virus
Simian Acquired Immunodeficiency Syndrome
Sensitivity and Specificity
Reproducibility of Results
Reference Standards
Protein Binding
Practice Guidelines as Topic
Microspheres
Macaca mulatta

Citation

APA

Chicago

ICMJE

MLA

NLM

Brown, E. P., Weiner, J. A., Lin, S., Natarajan, H., Normandin, E., Barouch, D. H., … Ackerman, M. E. (2018). Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV. J Immunol Methods, 455, 24–33. https://doi.org/10.1016/j.jim.2018.01.013

Brown, Eric P., Joshua A. Weiner, Shu Lin, Harini Natarajan, Erica Normandin, Dan H. Barouch, Galit Alter, Marcella Sarzotti-Kelsoe, and Margaret E. Ackerman. “Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV.” J Immunol Methods 455 (April 2018): 24–33. https://doi.org/10.1016/j.jim.2018.01.013.

Brown EP, Weiner JA, Lin S, Natarajan H, Normandin E, Barouch DH, et al. Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV. J Immunol Methods. 2018 Apr;455:24–33.

Brown, Eric P., et al. “Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV.” J Immunol Methods, vol. 455, Apr. 2018, pp. 24–33. Pubmed, doi:10.1016/j.jim.2018.01.013.

Brown EP, Weiner JA, Lin S, Natarajan H, Normandin E, Barouch DH, Alter G, Sarzotti-Kelsoe M, Ackerman ME. Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV. J Immunol Methods. 2018 Apr;455:24–33.

Published In

J Immunol Methods

DOI

10.1016/j.jim.2018.01.013

EISSN

1872-7905

Publication Date

April 2018

Volume

455

Start / End Page

24 / 33

Location

Netherlands

Related Subject Headings

Simian immunodeficiency virus
Simian Immunodeficiency Virus
Simian Acquired Immunodeficiency Syndrome
Sensitivity and Specificity
Reproducibility of Results
Reference Standards
Protein Binding
Practice Guidelines as Topic
Microspheres
Macaca mulatta