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De novo variants in the alternative exon 5 of SCN8A cause epileptic encephalopathy.

Publication ,  Journal Article
Epilepsy Genetics Initiative,
Published in: Genet Med
February 2018

PurposeAs part of the Epilepsy Genetics Initiative, we re-evaluated clinically generated exome sequence data from 54 epilepsy patients and their unaffected parents to identify molecular diagnoses not provided in the initial diagnostic interpretation.MethodsWe compiled and analyzed exome sequence data from 54 genetically undiagnosed trios using a validated analysis pipeline. We evaluated the significance of the genetic findings by reanalyzing sequence data generated at Ambry Genetics, and from a number of additional case and control cohorts.ResultsIn 54 previously undiagnosed trios, we identified two de novo missense variants in SCN8A in the highly expressed alternative exon 5 A-an exon only recently added to the Consensus Coding Sequence database. One additional undiagnosed epilepsy patient harboring a de novo variant in exon 5 A was found in the Ambry Genetics cohort. Missense variants in SCN8A exon 5 A are extremely rare in the population, further supporting the pathogenicity of the de novo alterations identified.ConclusionThese results expand the range of SCN8A variants in epileptic encephalopathy patients and illustrate the necessity of ongoing reanalysis of negative exome sequences, as advances in the knowledge of disease genes and their annotations will permit new diagnoses to be made.

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Published In

Genet Med

DOI

EISSN

1530-0366

Publication Date

February 2018

Volume

20

Issue

2

Start / End Page

275 / 281

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Phenotype
  • NAV1.6 Voltage-Gated Sodium Channel
  • Mutation
  • Male
  • Humans
  • Genotype
  • Genetics & Heredity
  • Genetic Testing
  • Genetic Association Studies
 

Citation

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Epilepsy Genetics Initiative, . (2018). De novo variants in the alternative exon 5 of SCN8A cause epileptic encephalopathy. Genet Med, 20(2), 275–281. https://doi.org/10.1038/gim.2017.100
Epilepsy Genetics Initiative, Patrick S. “De novo variants in the alternative exon 5 of SCN8A cause epileptic encephalopathy.Genet Med 20, no. 2 (February 2018): 275–81. https://doi.org/10.1038/gim.2017.100.
Epilepsy Genetics Initiative. De novo variants in the alternative exon 5 of SCN8A cause epileptic encephalopathy. Genet Med. 2018 Feb;20(2):275–81.
Epilepsy Genetics Initiative, Patrick S. “De novo variants in the alternative exon 5 of SCN8A cause epileptic encephalopathy.Genet Med, vol. 20, no. 2, Feb. 2018, pp. 275–81. Pubmed, doi:10.1038/gim.2017.100.
Epilepsy Genetics Initiative. De novo variants in the alternative exon 5 of SCN8A cause epileptic encephalopathy. Genet Med. 2018 Feb;20(2):275–281.

Published In

Genet Med

DOI

EISSN

1530-0366

Publication Date

February 2018

Volume

20

Issue

2

Start / End Page

275 / 281

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Phenotype
  • NAV1.6 Voltage-Gated Sodium Channel
  • Mutation
  • Male
  • Humans
  • Genotype
  • Genetics & Heredity
  • Genetic Testing
  • Genetic Association Studies