DNA, RNA Chemical Properties (Including Sequencing and Next-Generation Sequencing)
The chemistry and structure of nucleic acids are discussed with emphasis on the nucleobases including the use of nucleoside analogues as drugs. The importance of complementarity in nucleic acid function is discussed including presentation of a DNA melting curve. The development of DNA sequence analysis is presented starting with Maxam-Gilbert and Sanger dideoxy sequencing, Emphasis is placed on next-generation sequencing (next-gen sequencing) including library preparation and data analysis. Topics include sequence by synthesis including pyrosequencing. There is a brief mention of emerging technologies such as nanopore sequencing.