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Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly.

Publication ,  Journal Article
Mozhdehi, D; Luginbuhl, KM; Simon, JR; Dzuricky, M; Berger, R; Varol, HS; Huang, FC; Buehne, KL; Mayne, NR; Weitzhandler, I; Bonn, M ...
Published in: Nature chemistry
May 2018

Post-translational modification of proteins is a strategy widely used in biological systems. It expands the diversity of the proteome and allows for tailoring of both the function and localization of proteins within cells as well as the material properties of structural proteins and matrices. Despite their ubiquity in biology, with a few exceptions, the potential of post-translational modifications in biomaterials synthesis has remained largely untapped. As a proof of concept to demonstrate the feasibility of creating a genetically encoded biohybrid material through post-translational modification, we report here the generation of a family of three stimulus-responsive hybrid materials-fatty-acid-modified elastin-like polypeptides-using a one-pot recombinant expression and post-translational lipidation methodology. These hybrid biomaterials contain an amphiphilic domain, composed of a β-sheet-forming peptide that is post-translationally functionalized with a C14 alkyl chain, fused to a thermally responsive elastin-like polypeptide. They exhibit temperature-triggered hierarchical self-assembly across multiple length scales with varied structure and material properties that can be controlled at the sequence level.

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Published In

Nature chemistry

DOI

EISSN

1755-4349

ISSN

1755-4330

Publication Date

May 2018

Volume

10

Issue

5

Start / End Page

496 / 505

Related Subject Headings

  • Temperature
  • Protein Processing, Post-Translational
  • Peptides
  • Organic Chemistry
  • Microscopy, Electron, Transmission
  • Microscopy, Electron, Scanning
  • Lipids
  • Elastin
  • Cryoelectron Microscopy
  • Biocompatible Materials
 

Citation

APA
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ICMJE
MLA
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Mozhdehi, D., Luginbuhl, K. M., Simon, J. R., Dzuricky, M., Berger, R., Varol, H. S., … Chilkoti, A. (2018). Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly. Nature Chemistry, 10(5), 496–505. https://doi.org/10.1038/s41557-018-0005-z
Mozhdehi, Davoud, Kelli M. Luginbuhl, Joseph R. Simon, Michael Dzuricky, Rüdiger Berger, H Samet Varol, Fred C. Huang, et al. “Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly.Nature Chemistry 10, no. 5 (May 2018): 496–505. https://doi.org/10.1038/s41557-018-0005-z.
Mozhdehi D, Luginbuhl KM, Simon JR, Dzuricky M, Berger R, Varol HS, et al. Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly. Nature chemistry. 2018 May;10(5):496–505.
Mozhdehi, Davoud, et al. “Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly.Nature Chemistry, vol. 10, no. 5, May 2018, pp. 496–505. Epmc, doi:10.1038/s41557-018-0005-z.
Mozhdehi D, Luginbuhl KM, Simon JR, Dzuricky M, Berger R, Varol HS, Huang FC, Buehne KL, Mayne NR, Weitzhandler I, Bonn M, Parekh SH, Chilkoti A. Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly. Nature chemistry. 2018 May;10(5):496–505.

Published In

Nature chemistry

DOI

EISSN

1755-4349

ISSN

1755-4330

Publication Date

May 2018

Volume

10

Issue

5

Start / End Page

496 / 505

Related Subject Headings

  • Temperature
  • Protein Processing, Post-Translational
  • Peptides
  • Organic Chemistry
  • Microscopy, Electron, Transmission
  • Microscopy, Electron, Scanning
  • Lipids
  • Elastin
  • Cryoelectron Microscopy
  • Biocompatible Materials