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Sustained Transgene Expression via Hydrogel-Mediated Gene Transfer Results from Multiple Transfection Events.

Publication ,  Journal Article
Truong, NF; Segura, T
Published in: ACS biomaterials science & engineering
March 2018

Sustained delivery of therapeutic genes in vitro and in vivo has a wide range of applications in studying biology and in developing therapies for treating disease or repairing tissue. Nonviral vectors such as cationic polymers still present promising approaches; however, bolus transfection methods with polyethylenimine-based DNA polyplexes suffer from considerable levels of cytotoxicity and short-lived transgene expression levels. Here, we designed and characterized a hyaluronic acid-based porous hydrogel system for nonviral gene delivery by loading with surface-associated DNA polyplexes. With this, we observed tunable, enhanced, and sustained transgene expression over 30 days of cell culture with better cell viability and marked improvements over comparable bolus transfection techniques. Finally, we investigated mechanisms thought to be responsible for the sustained expression profile, finding that multiple transfection events are likely responsible for the observed sustained expression.

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Published In

ACS biomaterials science & engineering

DOI

EISSN

2373-9878

ISSN

2373-9878

Publication Date

March 2018

Volume

4

Issue

3

Start / End Page

981 / 987

Related Subject Headings

  • 4003 Biomedical engineering
  • 0903 Biomedical Engineering
 

Citation

APA
Chicago
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MLA
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Truong, N. F., & Segura, T. (2018). Sustained Transgene Expression via Hydrogel-Mediated Gene Transfer Results from Multiple Transfection Events. ACS Biomaterials Science & Engineering, 4(3), 981–987. https://doi.org/10.1021/acsbiomaterials.7b00957
Truong, Norman F., and Tatiana Segura. “Sustained Transgene Expression via Hydrogel-Mediated Gene Transfer Results from Multiple Transfection Events.ACS Biomaterials Science & Engineering 4, no. 3 (March 2018): 981–87. https://doi.org/10.1021/acsbiomaterials.7b00957.
Truong NF, Segura T. Sustained Transgene Expression via Hydrogel-Mediated Gene Transfer Results from Multiple Transfection Events. ACS biomaterials science & engineering. 2018 Mar;4(3):981–7.
Truong, Norman F., and Tatiana Segura. “Sustained Transgene Expression via Hydrogel-Mediated Gene Transfer Results from Multiple Transfection Events.ACS Biomaterials Science & Engineering, vol. 4, no. 3, Mar. 2018, pp. 981–87. Epmc, doi:10.1021/acsbiomaterials.7b00957.
Truong NF, Segura T. Sustained Transgene Expression via Hydrogel-Mediated Gene Transfer Results from Multiple Transfection Events. ACS biomaterials science & engineering. 2018 Mar;4(3):981–987.
Journal cover image

Published In

ACS biomaterials science & engineering

DOI

EISSN

2373-9878

ISSN

2373-9878

Publication Date

March 2018

Volume

4

Issue

3

Start / End Page

981 / 987

Related Subject Headings

  • 4003 Biomedical engineering
  • 0903 Biomedical Engineering