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Cross-talk Between Nitrate-Nitrite-NO and NO Synthase Pathways in Control of Vascular NO Homeostasis.

Publication ,  Journal Article
Carlström, M; Liu, M; Yang, T; Zollbrecht, C; Huang, L; Peleli, M; Borniquel, S; Kishikawa, H; Hezel, M; Persson, AEG; Weitzberg, E; Lundberg, JO
Published in: Antioxid Redox Signal
August 1, 2015

AIMS: Inorganic nitrate and nitrite from endogenous and dietary sources have emerged as alternative substrates for nitric oxide (NO) formation in addition to the classic L-arginine NO synthase (NOS)-dependent pathway. Here, we investigated a potential cross-talk between these two pathways in the regulation of vascular function. RESULTS: Long-term dietary supplementation with sodium nitrate (0.1 and 1 mmol kg(-1) day(-1)) in rats caused a reversible dose-dependent reduction in phosphorylated endothelial NOS (eNOS) (Ser1177) in aorta and a concomitant increase in phosphorylation at Thr495. Moreover, eNOS-dependent vascular responses were attenuated in vessels harvested from nitrate-treated mice or when nitrite was acutely added to control vessels. The citrulline-to-arginine ratio in plasma, as a measure of eNOS activity, was reduced in nitrate-treated rodents. Telemetry measurements revealed that a low dietary nitrate dose reduced blood pressure, whereas a higher dose was associated with a paradoxical elevation. Finally, plasma cyclic guanosine monophosphate increased in mice that were treated with a low dietary nitrate dose and decreased with a higher dose. INNOVATION AND CONCLUSIONS: These results demonstrate the existence of a cross-talk between the nitrate-nitrite-NO pathway and the NOS-dependent pathway in control of vascular NO homeostasis.

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Published In

Antioxid Redox Signal

DOI

EISSN

1557-7716

Publication Date

August 1, 2015

Volume

23

Issue

4

Start / End Page

295 / 306

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Phosphorylation
  • Nitrites
  • Nitric Oxide Synthase Type III
  • Nitric Oxide
  • Nitrates
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Metabolic Networks and Pathways
  • Male
 

Citation

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Carlström, M., Liu, M., Yang, T., Zollbrecht, C., Huang, L., Peleli, M., … Lundberg, J. O. (2015). Cross-talk Between Nitrate-Nitrite-NO and NO Synthase Pathways in Control of Vascular NO Homeostasis. Antioxid Redox Signal, 23(4), 295–306. https://doi.org/10.1089/ars.2013.5481
Carlström, Mattias, Ming Liu, Ting Yang, Christa Zollbrecht, Liyue Huang, Maria Peleli, Sara Borniquel, et al. “Cross-talk Between Nitrate-Nitrite-NO and NO Synthase Pathways in Control of Vascular NO Homeostasis.Antioxid Redox Signal 23, no. 4 (August 1, 2015): 295–306. https://doi.org/10.1089/ars.2013.5481.
Carlström M, Liu M, Yang T, Zollbrecht C, Huang L, Peleli M, et al. Cross-talk Between Nitrate-Nitrite-NO and NO Synthase Pathways in Control of Vascular NO Homeostasis. Antioxid Redox Signal. 2015 Aug 1;23(4):295–306.
Carlström, Mattias, et al. “Cross-talk Between Nitrate-Nitrite-NO and NO Synthase Pathways in Control of Vascular NO Homeostasis.Antioxid Redox Signal, vol. 23, no. 4, Aug. 2015, pp. 295–306. Pubmed, doi:10.1089/ars.2013.5481.
Carlström M, Liu M, Yang T, Zollbrecht C, Huang L, Peleli M, Borniquel S, Kishikawa H, Hezel M, Persson AEG, Weitzberg E, Lundberg JO. Cross-talk Between Nitrate-Nitrite-NO and NO Synthase Pathways in Control of Vascular NO Homeostasis. Antioxid Redox Signal. 2015 Aug 1;23(4):295–306.
Journal cover image

Published In

Antioxid Redox Signal

DOI

EISSN

1557-7716

Publication Date

August 1, 2015

Volume

23

Issue

4

Start / End Page

295 / 306

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Phosphorylation
  • Nitrites
  • Nitric Oxide Synthase Type III
  • Nitric Oxide
  • Nitrates
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Metabolic Networks and Pathways
  • Male