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Protein Corona in Response to Flow: Effect on Protein Concentration and Structure.

Publication ,  Journal Article
Jayaram, DT; Pustulka, SM; Mannino, RG; Lam, WA; Payne, CK
Published in: Biophysical journal
July 2018

Nanoparticles used in cellular applications encounter free serum proteins that adsorb onto the surface of the nanoparticle, forming a protein corona. This protein layer controls the interaction of nanoparticles with cells. For nanomedicine applications, it is important to consider how intravenous injection and the subsequent shear flow will affect the protein corona. Our goal was to determine if shear flow changed the composition of the protein corona and if these changes affected cellular binding. Colorimetric assays of protein concentration and gel electrophoresis demonstrate that polystyrene nanoparticles subjected to flow have a greater concentration of serum proteins adsorbed on the surface, especially plasminogen. Plasminogen, in the absence of nanoparticles, undergoes changes in structure in response to flow, characterized by fluorescence and circular dichroism spectroscopy. The protein-nanoparticle complexes formed from fetal bovine serum after flow had decreased cellular binding, as measured with flow cytometry. In addition to the relevance for nanomedicine, these results also highlight the technical challenges of protein corona studies. The composition of the protein corona was highly dependent on the initial mixing step: rocking, vortexing, or flow. Overall, these results reaffirm the importance of the protein corona in nanoparticle-cell interactions and point toward the challenges of predicting corona composition based on nanoparticle properties.

Duke Scholars

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

July 2018

Volume

115

Issue

2

Start / End Page

209 / 216

Related Subject Headings

  • Protein Corona
  • Polystyrenes
  • Plasminogen
  • Nanoparticles
  • Hydrodynamics
  • Humans
  • Hela Cells
  • HeLa Cells
  • Cattle
  • Biophysics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jayaram, D. T., Pustulka, S. M., Mannino, R. G., Lam, W. A., & Payne, C. K. (2018). Protein Corona in Response to Flow: Effect on Protein Concentration and Structure. Biophysical Journal, 115(2), 209–216. https://doi.org/10.1016/j.bpj.2018.02.036
Jayaram, Dhanya T., Samantha M. Pustulka, Robert G. Mannino, Wilbur A. Lam, and Christine K. Payne. “Protein Corona in Response to Flow: Effect on Protein Concentration and Structure.Biophysical Journal 115, no. 2 (July 2018): 209–16. https://doi.org/10.1016/j.bpj.2018.02.036.
Jayaram DT, Pustulka SM, Mannino RG, Lam WA, Payne CK. Protein Corona in Response to Flow: Effect on Protein Concentration and Structure. Biophysical journal. 2018 Jul;115(2):209–16.
Jayaram, Dhanya T., et al. “Protein Corona in Response to Flow: Effect on Protein Concentration and Structure.Biophysical Journal, vol. 115, no. 2, July 2018, pp. 209–16. Epmc, doi:10.1016/j.bpj.2018.02.036.
Jayaram DT, Pustulka SM, Mannino RG, Lam WA, Payne CK. Protein Corona in Response to Flow: Effect on Protein Concentration and Structure. Biophysical journal. 2018 Jul;115(2):209–216.
Journal cover image

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

July 2018

Volume

115

Issue

2

Start / End Page

209 / 216

Related Subject Headings

  • Protein Corona
  • Polystyrenes
  • Plasminogen
  • Nanoparticles
  • Hydrodynamics
  • Humans
  • Hela Cells
  • HeLa Cells
  • Cattle
  • Biophysics