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Molecular and biophysical analysis of apoptosis using a combined quantitative phase imaging and fluorescence resonance energy transfer microscope.

Publication ,  Journal Article
Eldridge, WJ; Hoballah, J; Wax, A
Published in: Journal of biophotonics
December 2018

Apoptotic mechanisms are often dysregulated in cancerous phenotypes. Additionally, many anticancer treatments induce apoptosis and necrosis, and the monitoring of this apoptotic activity can allow researchers to identify therapeutic efficiency. Here, we introduce a microscope which combines quantitative phase imaging (QPI) with the ability to detect molecular events via fluorescence (or Förster) resonance energy transfer (FRET). The system was applied to study cells undergoing apoptosis to correlate the onset of apoptotic enzyme activity as observed using a FRET-based apoptosis sensor with whole cell morphological changes analyzed via QPI. The QPI data showed changes in cell disorder strength during the initiation of apoptotic enzymatic activity.

Duke Scholars

Published In

Journal of biophotonics

DOI

EISSN

1864-0648

ISSN

1864-063X

Publication Date

December 2018

Volume

11

Issue

12

Start / End Page

e201800126

Related Subject Headings

  • Optoelectronics & Photonics
  • Microscopy, Fluorescence
  • Humans
  • Hela Cells
  • HeLa Cells
  • Fluorescence Resonance Energy Transfer
  • Enzyme Activation
  • Caspase 3
  • Biophysics
  • Apoptosis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Eldridge, W. J., Hoballah, J., & Wax, A. (2018). Molecular and biophysical analysis of apoptosis using a combined quantitative phase imaging and fluorescence resonance energy transfer microscope. Journal of Biophotonics, 11(12), e201800126. https://doi.org/10.1002/jbio.201800126
Eldridge, Will J., Jawad Hoballah, and Adam Wax. “Molecular and biophysical analysis of apoptosis using a combined quantitative phase imaging and fluorescence resonance energy transfer microscope.Journal of Biophotonics 11, no. 12 (December 2018): e201800126. https://doi.org/10.1002/jbio.201800126.
Eldridge, Will J., et al. “Molecular and biophysical analysis of apoptosis using a combined quantitative phase imaging and fluorescence resonance energy transfer microscope.Journal of Biophotonics, vol. 11, no. 12, Dec. 2018, p. e201800126. Epmc, doi:10.1002/jbio.201800126.
Journal cover image

Published In

Journal of biophotonics

DOI

EISSN

1864-0648

ISSN

1864-063X

Publication Date

December 2018

Volume

11

Issue

12

Start / End Page

e201800126

Related Subject Headings

  • Optoelectronics & Photonics
  • Microscopy, Fluorescence
  • Humans
  • Hela Cells
  • HeLa Cells
  • Fluorescence Resonance Energy Transfer
  • Enzyme Activation
  • Caspase 3
  • Biophysics
  • Apoptosis