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Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry.

Publication ,  Journal Article
Popescu, I; Pipeling, M; Akulian, J; McDyer, J
Published in: Methods Mol Biol
2014

Cytotoxic T lymphocytes (CTLs) are important constituents of the adaptive immune system. Development of CTLs are particularly important for bacterial and viral infections, in addition to tumor surveillance. Measuring T cell immune function is important in evaluating host defense, allergy, autoimmunity, transplant rejection, and tumor immunity. In these recent years it has become possible to measure multiple effector functions in a single cell such as cytokine, transcription factors, and cytolytic function. In addition these parameters can be evaluated in conjunction with cellular proliferation. In this chapter we detail these cellular based assays and the methods used to characterize and quantify both phenotype and function of CTL.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2014

Volume

1186

Start / End Page

21 / 47

Location

United States

Related Subject Headings

  • fas Receptor
  • T-Lymphocytes, Helper-Inducer
  • T-Lymphocytes, Cytotoxic
  • Staining and Labeling
  • Receptors, Antigen, T-Cell
  • Perforin
  • Microspheres
  • Lysosomal-Associated Membrane Protein 1
  • Lymphocyte Activation
  • Leukocyte Common Antigens
 

Citation

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Popescu, I., Pipeling, M., Akulian, J., & McDyer, J. (2014). Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry. Methods Mol Biol, 1186, 21–47. https://doi.org/10.1007/978-1-4939-1158-5_3
Popescu, Iulia, Matthew Pipeling, Jason Akulian, and John McDyer. “Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry.Methods Mol Biol 1186 (2014): 21–47. https://doi.org/10.1007/978-1-4939-1158-5_3.
Popescu I, Pipeling M, Akulian J, McDyer J. Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry. Methods Mol Biol. 2014;1186:21–47.
Popescu, Iulia, et al. “Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry.Methods Mol Biol, vol. 1186, 2014, pp. 21–47. Pubmed, doi:10.1007/978-1-4939-1158-5_3.
Popescu I, Pipeling M, Akulian J, McDyer J. Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry. Methods Mol Biol. 2014;1186:21–47.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2014

Volume

1186

Start / End Page

21 / 47

Location

United States

Related Subject Headings

  • fas Receptor
  • T-Lymphocytes, Helper-Inducer
  • T-Lymphocytes, Cytotoxic
  • Staining and Labeling
  • Receptors, Antigen, T-Cell
  • Perforin
  • Microspheres
  • Lysosomal-Associated Membrane Protein 1
  • Lymphocyte Activation
  • Leukocyte Common Antigens