Spotiton: New features and applications.
Publication
, Journal Article
Dandey, VP; Wei, H; Zhang, Z; Tan, YZ; Acharya, P; Eng, ET; Rice, WJ; Kahn, PA; Potter, CS; Carragher, B
Published in: J Struct Biol
May 2018
We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ∼3 Å resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples.
Duke Scholars
Published In
J Struct Biol
DOI
EISSN
1095-8657
Publication Date
May 2018
Volume
202
Issue
2
Start / End Page
161 / 169
Location
United States
Related Subject Headings
- Vitrification
- Specimen Handling
- Robotics
- Optical Tweezers
- Nanowires
- Cryoelectron Microscopy
- Biophysics
- 3101 Biochemistry and cell biology
- 0608 Zoology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Dandey, V. P., Wei, H., Zhang, Z., Tan, Y. Z., Acharya, P., Eng, E. T., … Carragher, B. (2018). Spotiton: New features and applications. J Struct Biol, 202(2), 161–169. https://doi.org/10.1016/j.jsb.2018.01.002
Dandey, Venkata P., Hui Wei, Zhening Zhang, Yong Zi Tan, Priyamvada Acharya, Edward T. Eng, William J. Rice, Peter A. Kahn, Clinton S. Potter, and Bridget Carragher. “Spotiton: New features and applications.” J Struct Biol 202, no. 2 (May 2018): 161–69. https://doi.org/10.1016/j.jsb.2018.01.002.
Dandey VP, Wei H, Zhang Z, Tan YZ, Acharya P, Eng ET, et al. Spotiton: New features and applications. J Struct Biol. 2018 May;202(2):161–9.
Dandey, Venkata P., et al. “Spotiton: New features and applications.” J Struct Biol, vol. 202, no. 2, May 2018, pp. 161–69. Pubmed, doi:10.1016/j.jsb.2018.01.002.
Dandey VP, Wei H, Zhang Z, Tan YZ, Acharya P, Eng ET, Rice WJ, Kahn PA, Potter CS, Carragher B. Spotiton: New features and applications. J Struct Biol. 2018 May;202(2):161–169.
Published In
J Struct Biol
DOI
EISSN
1095-8657
Publication Date
May 2018
Volume
202
Issue
2
Start / End Page
161 / 169
Location
United States
Related Subject Headings
- Vitrification
- Specimen Handling
- Robotics
- Optical Tweezers
- Nanowires
- Cryoelectron Microscopy
- Biophysics
- 3101 Biochemistry and cell biology
- 0608 Zoology
- 0601 Biochemistry and Cell Biology