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Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation.

Publication ,  Journal Article
Wu, M; Huang, P-H; Zhang, R; Mao, Z; Chen, C; Kemeny, G; Li, P; Lee, AV; Gyanchandani, R; Armstrong, AJ; Dao, M; Suresh, S; Huang, TJ
Published in: Small
August 2018

The study of circulating tumor cells (CTCs) offers pathways to develop new diagnostic and prognostic biomarkers that benefit cancer treatments. In order to fully exploit and interpret the information provided by CTCs, the development of a platform is reported that integrates acoustics and microfluidics to isolate rare CTCs from peripheral blood in high throughput while preserving their structural, biological, and functional integrity. Cancer cells are first isolated from leukocytes with a throughput of 7.5 mL h-1 , achieving a recovery rate of at least 86% while maintaining the cells' ability to proliferate. High-throughput acoustic separation enables statistical analysis of isolated CTCs from prostate cancer patients to be performed to determine their size distribution and phenotypic heterogeneity for a range of biomarkers, including the visualization of CTCs with a loss of expression for the prostate specific membrane antigen. The method also enables the isolation of even rarer, but clinically important, CTC clusters.

Duke Scholars

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Published In

Small

DOI

EISSN

1613-6829

Publication Date

August 2018

Volume

14

Issue

32

Start / End Page

e1801131

Location

Germany

Related Subject Headings

  • Rheology
  • Prostatic Neoplasms
  • Phenotype
  • Numerical Analysis, Computer-Assisted
  • Neoplastic Cells, Circulating
  • Nanoscience & Nanotechnology
  • Male
  • Leukocytes
  • Humans
  • High-Throughput Screening Assays
 

Citation

APA
Chicago
ICMJE
MLA
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Wu, M., Huang, P.-H., Zhang, R., Mao, Z., Chen, C., Kemeny, G., … Huang, T. J. (2018). Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation. Small, 14(32), e1801131. https://doi.org/10.1002/smll.201801131
Wu, Mengxi, Po-Hsun Huang, Rui Zhang, Zhangming Mao, Chuyi Chen, Gabor Kemeny, Peng Li, et al. “Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation.Small 14, no. 32 (August 2018): e1801131. https://doi.org/10.1002/smll.201801131.
Wu M, Huang P-H, Zhang R, Mao Z, Chen C, Kemeny G, et al. Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation. Small. 2018 Aug;14(32):e1801131.
Wu, Mengxi, et al. “Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation.Small, vol. 14, no. 32, Aug. 2018, p. e1801131. Pubmed, doi:10.1002/smll.201801131.
Wu M, Huang P-H, Zhang R, Mao Z, Chen C, Kemeny G, Li P, Lee AV, Gyanchandani R, Armstrong AJ, Dao M, Suresh S, Huang TJ. Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation. Small. 2018 Aug;14(32):e1801131.

Published In

Small

DOI

EISSN

1613-6829

Publication Date

August 2018

Volume

14

Issue

32

Start / End Page

e1801131

Location

Germany

Related Subject Headings

  • Rheology
  • Prostatic Neoplasms
  • Phenotype
  • Numerical Analysis, Computer-Assisted
  • Neoplastic Cells, Circulating
  • Nanoscience & Nanotechnology
  • Male
  • Leukocytes
  • Humans
  • High-Throughput Screening Assays