Optical Control of CRISPR/Cas9 Gene Editing.
Publication
, Journal Article
Hemphill, J; Borchardt, EK; Brown, K; Asokan, A; Deiters, A
Published in: J Am Chem Soc
May 6, 2015
The CRISPR/Cas9 system has emerged as an important tool in biomedical research for a wide range of applications, with significant potential for genome engineering and gene therapy. In order to achieve conditional control of the CRISPR/Cas9 system, a genetically encoded light-activated Cas9 was engineered through the site-specific installation of a caged lysine amino acid. Several potential lysine residues were identified as viable caging sites that can be modified to optically control Cas9 function, as demonstrated through optical activation and deactivation of both exogenous and endogenous gene function.
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Published In
J Am Chem Soc
DOI
EISSN
1520-5126
Publication Date
May 6, 2015
Volume
137
Issue
17
Start / End Page
5642 / 5645
Location
United States
Related Subject Headings
- RNA Editing
- Molecular Structure
- Models, Molecular
- Lysine
- Humans
- HEK293 Cells
- Genetic Engineering
- General Chemistry
- Clustered Regularly Interspaced Short Palindromic Repeats
- CRISPR-Cas Systems
Citation
APA
Chicago
ICMJE
MLA
NLM
Hemphill, J., Borchardt, E. K., Brown, K., Asokan, A., & Deiters, A. (2015). Optical Control of CRISPR/Cas9 Gene Editing. J Am Chem Soc, 137(17), 5642–5645. https://doi.org/10.1021/ja512664v
Hemphill, James, Erin K. Borchardt, Kalyn Brown, Aravind Asokan, and Alexander Deiters. “Optical Control of CRISPR/Cas9 Gene Editing.” J Am Chem Soc 137, no. 17 (May 6, 2015): 5642–45. https://doi.org/10.1021/ja512664v.
Hemphill J, Borchardt EK, Brown K, Asokan A, Deiters A. Optical Control of CRISPR/Cas9 Gene Editing. J Am Chem Soc. 2015 May 6;137(17):5642–5.
Hemphill, James, et al. “Optical Control of CRISPR/Cas9 Gene Editing.” J Am Chem Soc, vol. 137, no. 17, May 2015, pp. 5642–45. Pubmed, doi:10.1021/ja512664v.
Hemphill J, Borchardt EK, Brown K, Asokan A, Deiters A. Optical Control of CRISPR/Cas9 Gene Editing. J Am Chem Soc. 2015 May 6;137(17):5642–5645.
Published In
J Am Chem Soc
DOI
EISSN
1520-5126
Publication Date
May 6, 2015
Volume
137
Issue
17
Start / End Page
5642 / 5645
Location
United States
Related Subject Headings
- RNA Editing
- Molecular Structure
- Models, Molecular
- Lysine
- Humans
- HEK293 Cells
- Genetic Engineering
- General Chemistry
- Clustered Regularly Interspaced Short Palindromic Repeats
- CRISPR-Cas Systems