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Peptide affinity reagents for AAV capsid recognition and purification.

Publication ,  Journal Article
Pulicherla, N; Asokan, A
Published in: Gene Ther
October 2011
Published version (DOI) Link to item

We report the discovery of AAV capsid-binding peptides identified through phage panning. The heptapeptide motif GYVSRHP selectively recognized AAV serotype 8 capsids and blocked transduction in vitro. Recombinant AAV8 vectors were purified directly from crude cell lysate and supernatant through sequential application of peptide affinity and anion exchange chromatography. Peptide affinity reagents may serve as useful alternatives to monoclonal antibodies in AAV capsid recognition, and offer readily scalable solutions for purification of clinical grade AAV vectors.

Duke Scholars

Aravind Asokan
Author Aravind Asokan Surgery, Surgical Sciences
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Published In

Gene Ther

DOI

10.1038/gt.2011.46

EISSN

1476-5462

Publication Date

October 2011

Volume

18

Issue

10

Start / End Page

1020 / 1024

Location

England

Related Subject Headings

  • Peptides
  • Humans
  • Genetic Vectors
  • Dependovirus
  • DNA Primers
  • Chromatography, Ion Exchange
  • Chromatography, Affinity
  • Cell Line
  • Capsid
  • Biotechnology
 

Citation

APA
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MLA
NLM
Pulicherla, N., & Asokan, A. (2011). Peptide affinity reagents for AAV capsid recognition and purification. Gene Ther, 18(10), 1020–1024. https://doi.org/10.1038/gt.2011.46
Pulicherla, N., and A. Asokan. “Peptide affinity reagents for AAV capsid recognition and purification.” Gene Ther 18, no. 10 (October 2011): 1020–24. https://doi.org/10.1038/gt.2011.46.
Pulicherla N, Asokan A. Peptide affinity reagents for AAV capsid recognition and purification. Gene Ther. 2011 Oct;18(10):1020–4.
Pulicherla, N., and A. Asokan. “Peptide affinity reagents for AAV capsid recognition and purification.” Gene Ther, vol. 18, no. 10, Oct. 2011, pp. 1020–24. Pubmed, doi:10.1038/gt.2011.46.
Pulicherla N, Asokan A. Peptide affinity reagents for AAV capsid recognition and purification. Gene Ther. 2011 Oct;18(10):1020–1024.

Published In

Gene Ther

DOI

10.1038/gt.2011.46

EISSN

1476-5462

Publication Date

October 2011

Volume

18

Issue

10

Start / End Page

1020 / 1024

Location

England

Related Subject Headings

  • Peptides
  • Humans
  • Genetic Vectors
  • Dependovirus
  • DNA Primers
  • Chromatography, Ion Exchange
  • Chromatography, Affinity
  • Cell Line
  • Capsid
  • Biotechnology