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The isoforms of phospholipase C-gamma are differentially used by distinct human NK activating receptors.

Publication ,  Journal Article
Upshaw, JL; Schoon, RA; Dick, CJ; Billadeau, DD; Leibson, PJ
Published in: J Immunol
July 1, 2005

The two isoforms of phospholipase C (PLC)-gamma couple immune recognition receptors to important calcium- and protein kinase C-dependent cellular functions. It has been assumed that PLC-gamma1 and PLC-gamma2 have redundant functions and that the receptors can use whichever PLC-gamma isoform is preferentially expressed in a cell of a given hemopoietic lineage. In this study, we demonstrate that ITAM-containing immune recognition receptors can use either PLC-gamma1 or PLC-gamma2, whereas the novel NK cell-activating receptor NKG2D preferentially couples to PLC-gamma2. Experimental models evaluating signals from either endogenous receptors (FcR vs NKG2D-DAP10) or ectopically expressed chimeric receptors (with ITAM-containing cytoplasmic tails vs DAP10-containing cytoplasmic tails) demonstrate that PLC-gamma1 and PLC-gamma2 both regulate the functions of ITAM-containing receptors, whereas only PLC-gamma2 regulates the function of DAP10-coupled receptors. These data suggest that specific immune recognition receptors can differentially couple to the two isoforms of PLC-gamma. More broadly, these observations reveal a basis for selectively targeting the functions initiated by distinct immune recognition receptors.

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Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

July 1, 2005

Volume

175

Issue

1

Start / End Page

213 / 218

Location

United States

Related Subject Headings

  • Tyrosine
  • Type C Phospholipases
  • T-Lymphocytes, Cytotoxic
  • Recombinant Fusion Proteins
  • Receptors, Natural Killer Cell
  • Receptors, Immunologic
  • Receptors, Fc
  • Phosphorylation
  • Phospholipase C gamma
  • NK Cell Lectin-Like Receptor Subfamily K
 

Citation

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Upshaw, J. L., Schoon, R. A., Dick, C. J., Billadeau, D. D., & Leibson, P. J. (2005). The isoforms of phospholipase C-gamma are differentially used by distinct human NK activating receptors. J Immunol, 175(1), 213–218. https://doi.org/10.4049/jimmunol.175.1.213
Upshaw, Jadee L., Renee A. Schoon, Christopher J. Dick, Daniel D. Billadeau, and Paul J. Leibson. “The isoforms of phospholipase C-gamma are differentially used by distinct human NK activating receptors.J Immunol 175, no. 1 (July 1, 2005): 213–18. https://doi.org/10.4049/jimmunol.175.1.213.
Upshaw JL, Schoon RA, Dick CJ, Billadeau DD, Leibson PJ. The isoforms of phospholipase C-gamma are differentially used by distinct human NK activating receptors. J Immunol. 2005 Jul 1;175(1):213–8.
Upshaw, Jadee L., et al. “The isoforms of phospholipase C-gamma are differentially used by distinct human NK activating receptors.J Immunol, vol. 175, no. 1, July 2005, pp. 213–18. Pubmed, doi:10.4049/jimmunol.175.1.213.
Upshaw JL, Schoon RA, Dick CJ, Billadeau DD, Leibson PJ. The isoforms of phospholipase C-gamma are differentially used by distinct human NK activating receptors. J Immunol. 2005 Jul 1;175(1):213–218.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

July 1, 2005

Volume

175

Issue

1

Start / End Page

213 / 218

Location

United States

Related Subject Headings

  • Tyrosine
  • Type C Phospholipases
  • T-Lymphocytes, Cytotoxic
  • Recombinant Fusion Proteins
  • Receptors, Natural Killer Cell
  • Receptors, Immunologic
  • Receptors, Fc
  • Phosphorylation
  • Phospholipase C gamma
  • NK Cell Lectin-Like Receptor Subfamily K