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Identification and functional study of a mild allele of SlDELLA gene conferring the potential for improved yield in tomato.

Publication ,  Journal Article
Shinozaki, Y; Ezura, K; Hu, J; Okabe, Y; Bénard, C; Prodhomme, D; Gibon, Y; Sun, T-P; Ezura, H; Ariizumi, T
Published in: Scientific reports
August 2018

Parthenocarpy, or pollination-independent fruit set, is an attractive trait for fruit production and can be induced by increased responses to the phytohormone gibberellin (GA), which regulates diverse aspects of plant development. GA signaling in plants is negatively regulated by DELLA proteins. A loss-of-function mutant of tomato DELLA (SlDELLA), procera (pro) thus exhibits enhanced GA-response phenotypes including parthenocarpy, although the pro mutation also confers some disadvantages for practical breeding. This study identified a new milder hypomorphic allele of SlDELLA, procera-2 (pro-2), which showed weaker GA-response phenotypes than pro. The pro-2 mutant contains a single nucleotide substitution, corresponding to a single amino acid substitution in the SAW subdomain of the SlDELLA. Accumulation of the mutated SlDELLA transcripts in wild-type (WT) resulted in parthenocarpy, while introduction of intact SlDELLA into pro-2 rescued mutant phenotypes. Yeast two-hybrid assays revealed that SlDELLA interacted with three tomato homologues of GID1 GA receptors with increasing affinity upon GA treatment, while their interactions were reduced by the pro and pro-2 mutations. Both pro and pro-2 mutants produced higher fruit yields under high temperature conditions, which were resulted from higher fruit set efficiency, demonstrating the potential for genetic parthenocarpy to improve yield under adverse environmental conditions.

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Published In

Scientific reports

DOI

EISSN

2045-2322

ISSN

2045-2322

Publication Date

August 2018

Volume

8

Issue

1

Start / End Page

12043

Related Subject Headings

  • Triazoles
  • Solanum lycopersicum
  • Receptors, Cell Surface
  • Polymorphism, Single Nucleotide
  • Plant Proteins
  • Plant Growth Regulators
  • Gibberellins
  • Gene Expression Regulation, Plant
  • Fruit
  • Amino Acid Substitution
 

Citation

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Shinozaki, Y., Ezura, K., Hu, J., Okabe, Y., Bénard, C., Prodhomme, D., … Ariizumi, T. (2018). Identification and functional study of a mild allele of SlDELLA gene conferring the potential for improved yield in tomato. Scientific Reports, 8(1), 12043. https://doi.org/10.1038/s41598-018-30502-w
Shinozaki, Yoshihito, Kentaro Ezura, Jianhong Hu, Yoshihiro Okabe, Camille Bénard, Duyen Prodhomme, Yves Gibon, Tai-Ping Sun, Hiroshi Ezura, and Tohru Ariizumi. “Identification and functional study of a mild allele of SlDELLA gene conferring the potential for improved yield in tomato.Scientific Reports 8, no. 1 (August 2018): 12043. https://doi.org/10.1038/s41598-018-30502-w.
Shinozaki Y, Ezura K, Hu J, Okabe Y, Bénard C, Prodhomme D, et al. Identification and functional study of a mild allele of SlDELLA gene conferring the potential for improved yield in tomato. Scientific reports. 2018 Aug;8(1):12043.
Shinozaki, Yoshihito, et al. “Identification and functional study of a mild allele of SlDELLA gene conferring the potential for improved yield in tomato.Scientific Reports, vol. 8, no. 1, Aug. 2018, p. 12043. Epmc, doi:10.1038/s41598-018-30502-w.
Shinozaki Y, Ezura K, Hu J, Okabe Y, Bénard C, Prodhomme D, Gibon Y, Sun T-P, Ezura H, Ariizumi T. Identification and functional study of a mild allele of SlDELLA gene conferring the potential for improved yield in tomato. Scientific reports. 2018 Aug;8(1):12043.

Published In

Scientific reports

DOI

EISSN

2045-2322

ISSN

2045-2322

Publication Date

August 2018

Volume

8

Issue

1

Start / End Page

12043

Related Subject Headings

  • Triazoles
  • Solanum lycopersicum
  • Receptors, Cell Surface
  • Polymorphism, Single Nucleotide
  • Plant Proteins
  • Plant Growth Regulators
  • Gibberellins
  • Gene Expression Regulation, Plant
  • Fruit
  • Amino Acid Substitution