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Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement.

Publication ,  Journal Article
Parsons, JB; Frank, S; Bhella, D; Liang, M; Prentice, MB; Mulvihill, DP; Warren, MJ
Published in: Mol Cell
April 23, 2010

Compartmentalization is an important process, since it allows the segregation of metabolic activities and, in the era of synthetic biology, represents an important tool by which defined microenvironments can be created for specific metabolic functions. Indeed, some bacteria make specialized proteinaceous metabolic compartments called bacterial microcompartments (BMCs) or metabolosomes. Here we demonstrate that the shell of the metabolosome (representing an empty BMC) can be produced within E. coli cells by the coordinated expression of genes encoding structural proteins. A plethora of diverse structures can be generated by changing the expression profile of these genes, including the formation of large axial filaments that interfere with septation. Fusing GFP to PduC, PduD, or PduV, none of which are shell proteins, allows regiospecific targeting of the reporter group to the empty BMC. Live cell imaging provides unexpected evidence of filament-associated BMC movement within the cell in the presence of PduV.

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Published In

Mol Cell

DOI

EISSN

1097-4164

Publication Date

April 23, 2010

Volume

38

Issue

2

Start / End Page

305 / 315

Location

United States

Related Subject Headings

  • Organelles
  • Genes, Bacterial
  • Escherichia coli
  • Developmental Biology
  • Cloning, Molecular
  • Bacterial Proteins
  • Bacteria
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
 

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Parsons, J. B., Frank, S., Bhella, D., Liang, M., Prentice, M. B., Mulvihill, D. P., & Warren, M. J. (2010). Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement. Mol Cell, 38(2), 305–315. https://doi.org/10.1016/j.molcel.2010.04.008
Parsons, Joshua B., Stefanie Frank, David Bhella, Mingzhi Liang, Michael B. Prentice, Daniel P. Mulvihill, and Martin J. Warren. “Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement.Mol Cell 38, no. 2 (April 23, 2010): 305–15. https://doi.org/10.1016/j.molcel.2010.04.008.
Parsons JB, Frank S, Bhella D, Liang M, Prentice MB, Mulvihill DP, et al. Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement. Mol Cell. 2010 Apr 23;38(2):305–15.
Parsons, Joshua B., et al. “Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement.Mol Cell, vol. 38, no. 2, Apr. 2010, pp. 305–15. Pubmed, doi:10.1016/j.molcel.2010.04.008.
Parsons JB, Frank S, Bhella D, Liang M, Prentice MB, Mulvihill DP, Warren MJ. Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement. Mol Cell. 2010 Apr 23;38(2):305–315.
Journal cover image

Published In

Mol Cell

DOI

EISSN

1097-4164

Publication Date

April 23, 2010

Volume

38

Issue

2

Start / End Page

305 / 315

Location

United States

Related Subject Headings

  • Organelles
  • Genes, Bacterial
  • Escherichia coli
  • Developmental Biology
  • Cloning, Molecular
  • Bacterial Proteins
  • Bacteria
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences