Skip to main content

The Drosophila afadin homologue Canoe regulates linkage of the actin cytoskeleton to adherens junctions during apical constriction.

Publication ,  Journal Article
Sawyer, JK; Harris, NJ; Slep, KC; Gaul, U; Peifer, M
Published in: J Cell Biol
July 13, 2009

Cadherin-based adherens junctions (AJs) mediate cell adhesion and regulate cell shape change. The nectin-afadin complex also localizes to AJs and links to the cytoskeleton. Mammalian afadin has been suggested to be essential for adhesion and polarity establishment, but its mechanism of action is unclear. In contrast, Drosophila melanogaster's afadin homologue Canoe (Cno) has suggested roles in signal transduction during morphogenesis. We completely removed Cno from embryos, testing these hypotheses. Surprisingly, Cno is not essential for AJ assembly or for AJ maintenance in many tissues. However, morphogenesis is impaired from the start. Apical constriction of mesodermal cells initiates but is not completed. The actomyosin cytoskeleton disconnects from AJs, uncoupling actomyosin constriction and cell shape change. Cno has multiple direct interactions with AJ proteins, but is not a core part of the cadherin-catenin complex. Instead, Cno localizes to AJs by a Rap1- and actin-dependent mechanism. These data suggest that Cno regulates linkage between AJs and the actin cytoskeleton during morphogenesis.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Cell Biol

DOI

EISSN

1540-8140

Publication Date

July 13, 2009

Volume

186

Issue

1

Start / End Page

57 / 73

Location

United States

Related Subject Headings

  • rap1 GTP-Binding Proteins
  • alpha Catenin
  • Sequence Homology, Amino Acid
  • Rabbits
  • Protein Transport
  • Protein Binding
  • Organ Specificity
  • Mutation
  • Morphogenesis
  • Microfilament Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sawyer, J. K., Harris, N. J., Slep, K. C., Gaul, U., & Peifer, M. (2009). The Drosophila afadin homologue Canoe regulates linkage of the actin cytoskeleton to adherens junctions during apical constriction. J Cell Biol, 186(1), 57–73. https://doi.org/10.1083/jcb.200904001
Sawyer, Jessica K., Nathan J. Harris, Kevin C. Slep, Ulrike Gaul, and Mark Peifer. “The Drosophila afadin homologue Canoe regulates linkage of the actin cytoskeleton to adherens junctions during apical constriction.J Cell Biol 186, no. 1 (July 13, 2009): 57–73. https://doi.org/10.1083/jcb.200904001.
Sawyer JK, Harris NJ, Slep KC, Gaul U, Peifer M. The Drosophila afadin homologue Canoe regulates linkage of the actin cytoskeleton to adherens junctions during apical constriction. J Cell Biol. 2009 Jul 13;186(1):57–73.
Sawyer, Jessica K., et al. “The Drosophila afadin homologue Canoe regulates linkage of the actin cytoskeleton to adherens junctions during apical constriction.J Cell Biol, vol. 186, no. 1, July 2009, pp. 57–73. Pubmed, doi:10.1083/jcb.200904001.
Sawyer JK, Harris NJ, Slep KC, Gaul U, Peifer M. The Drosophila afadin homologue Canoe regulates linkage of the actin cytoskeleton to adherens junctions during apical constriction. J Cell Biol. 2009 Jul 13;186(1):57–73.

Published In

J Cell Biol

DOI

EISSN

1540-8140

Publication Date

July 13, 2009

Volume

186

Issue

1

Start / End Page

57 / 73

Location

United States

Related Subject Headings

  • rap1 GTP-Binding Proteins
  • alpha Catenin
  • Sequence Homology, Amino Acid
  • Rabbits
  • Protein Transport
  • Protein Binding
  • Organ Specificity
  • Mutation
  • Morphogenesis
  • Microfilament Proteins