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Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7.

Publication ,  Journal Article
Lo, Y-H; Sobhany, M; Hsu, AL; Ford, BL; Krahn, JM; Borgnia, MJ; Stanley, RE
Published in: Nat Commun
January 31, 2019

Rix7 is an essential type II AAA-ATPase required for the formation of the large ribosomal subunit. Rix7 has been proposed to utilize the power of ATP hydrolysis to drive the removal of assembly factors from pre-60S particles, but the mechanism of release is unknown. Rix7's mammalian homolog, NVL2 has been linked to cancer and mental illness disorders, highlighting the need to understand the molecular mechanisms of this essential machine. Here we report the cryo-EM reconstruction of the tandem AAA domains of Rix7 which form an asymmetric stacked homohexameric ring. We trapped Rix7 with a polypeptide in the central channel, revealing Rix7's role as a molecular unfoldase. The structure establishes that type II AAA-ATPases lacking the aromatic-hydrophobic motif within the first AAA domain can engage a substrate throughout the entire central channel. The structure also reveals that Rix7 contains unique post-α7 insertions within both AAA domains important for Rix7 function.

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Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

January 31, 2019

Volume

10

Issue

1

Start / End Page

513

Location

England

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Ribosomal Proteins
  • RNA, Ribosomal
  • Nuclear Proteins
  • Cryoelectron Microscopy
  • Adenosine Triphosphatases
  • ATPases Associated with Diverse Cellular Activities
 

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Lo, Y.-H., Sobhany, M., Hsu, A. L., Ford, B. L., Krahn, J. M., Borgnia, M. J., & Stanley, R. E. (2019). Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7. Nat Commun, 10(1), 513. https://doi.org/10.1038/s41467-019-08373-0
Lo, Yu-Hua, Mack Sobhany, Allen L. Hsu, Brittany L. Ford, Juno M. Krahn, Mario J. Borgnia, and Robin E. Stanley. “Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7.Nat Commun 10, no. 1 (January 31, 2019): 513. https://doi.org/10.1038/s41467-019-08373-0.
Lo Y-H, Sobhany M, Hsu AL, Ford BL, Krahn JM, Borgnia MJ, et al. Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7. Nat Commun. 2019 Jan 31;10(1):513.
Lo, Yu-Hua, et al. “Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7.Nat Commun, vol. 10, no. 1, Jan. 2019, p. 513. Pubmed, doi:10.1038/s41467-019-08373-0.
Lo Y-H, Sobhany M, Hsu AL, Ford BL, Krahn JM, Borgnia MJ, Stanley RE. Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7. Nat Commun. 2019 Jan 31;10(1):513.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

January 31, 2019

Volume

10

Issue

1

Start / End Page

513

Location

England

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Ribosomal Proteins
  • RNA, Ribosomal
  • Nuclear Proteins
  • Cryoelectron Microscopy
  • Adenosine Triphosphatases
  • ATPases Associated with Diverse Cellular Activities