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Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells.

Publication ,  Journal Article
Pipó-Ollé, E; Walke, P; Notabi, MK; El-Houri, RB; Østergaard Andersen, M; Needham, D; Arnspang, EC
Published in: Journal of visualized experiments : JoVE
February 2019

Nanoparticles are the focus of an increased interest in drug delivery systems for cancer therapy. Lipid-coated nanoparticles are inspired in structure and size by low-density lipoproteins (LDLs) because cancer cells have an increased need for cholesterol to proliferate, and this has been exploited as a mechanism for delivering anticancer drugs to cancer cells. Moreover, depending on drug chemistry, encapsulating the drug can be advantageous to avoid degradation of the drug during circulation in vivo. Therefore, in this study, this design is used to fabricate lipid-coated nanoparticles of the anticancer drug falcarindiol, providing a potential new delivery system of falcarindiol in order to stabilize its chemical structure against degradation and improve its uptake by tumors. Falcarindiol nanoparticles, with a phospholipid and cholesterol monolayer encapsulating the purified drug core of the particle, were designed. The lipid monolayer coating consists of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol (Chol), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE PEG 2000) along with the fluorescent label DiI (molar ratios of 43:50:5:2). The nanoparticles are fabricated using the rapid injection method, which is a fast and simple technique to precipitate nanoparticles by good-solvent for anti-solvent exchange. It consists of a rapid injection of an ethanol solution containing the nanoparticle components into an aqueous phase. The size of the fluorescent nanoparticles is measured using dynamic light scattering (DLS) at 74.1 ± 6.7 nm. The uptake of the nanoparticles is tested in human mesenchymal stem cells (hMSCs) and imaged using fluorescence and confocal microscopy. The uptake of the nanoparticles is observed in hMSCs, suggesting the potential for such a stable drug delivery system for falcarindiol.

Duke Scholars

Published In

Journal of visualized experiments : JoVE

DOI

EISSN

1940-087X

ISSN

1940-087X

Publication Date

February 2019

Issue

144

Related Subject Headings

  • Polyethylene Glycols
  • Phosphatidylethanolamines
  • Nanoparticles
  • Mesenchymal Stem Cells
  • Humans
  • Fatty Alcohols
  • Drug Delivery Systems
  • Diynes
  • 3101 Biochemistry and cell biology
  • 1702 Cognitive Sciences
 

Citation

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Pipó-Ollé, E., Walke, P., Notabi, M. K., El-Houri, R. B., Østergaard Andersen, M., Needham, D., & Arnspang, E. C. (2019). Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells. Journal of Visualized Experiments : JoVE, (144). https://doi.org/10.3791/59094
Pipó-Ollé, Emma, Prasad Walke, Martine K. Notabi, Rime B. El-Houri, Morten Østergaard Andersen, David Needham, and Eva C. Arnspang. “Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells.Journal of Visualized Experiments : JoVE, no. 144 (February 2019). https://doi.org/10.3791/59094.
Pipó-Ollé E, Walke P, Notabi MK, El-Houri RB, Østergaard Andersen M, Needham D, et al. Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells. Journal of visualized experiments : JoVE. 2019 Feb;(144).
Pipó-Ollé, Emma, et al. “Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells.Journal of Visualized Experiments : JoVE, no. 144, Feb. 2019. Epmc, doi:10.3791/59094.
Pipó-Ollé E, Walke P, Notabi MK, El-Houri RB, Østergaard Andersen M, Needham D, Arnspang EC. Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells. Journal of visualized experiments : JoVE. 2019 Feb;(144).

Published In

Journal of visualized experiments : JoVE

DOI

EISSN

1940-087X

ISSN

1940-087X

Publication Date

February 2019

Issue

144

Related Subject Headings

  • Polyethylene Glycols
  • Phosphatidylethanolamines
  • Nanoparticles
  • Mesenchymal Stem Cells
  • Humans
  • Fatty Alcohols
  • Drug Delivery Systems
  • Diynes
  • 3101 Biochemistry and cell biology
  • 1702 Cognitive Sciences