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A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing.

Publication ,  Journal Article
Jones, EM; Jajoo, R; Cancilla, D; Lubock, NB; Wang, J; Satyadi, M; Chong, R; de March, C; Bloom, JS; Matsunami, H; Kosuri, S
Published in: Cell Syst
March 27, 2019

G protein-coupled receptors (GPCRs) are central to how mammalian cells sense and respond to chemicals. Mammalian olfactory receptors (ORs), the largest family of GPCRs, mediate the sense of smell through activation by small molecules, though for most bonafide ligands, they have not been identified. Here, we introduce a platform to screen large chemical panels against multiplexed GPCR libraries using next-generation sequencing of barcoded genetic reporters in stably engineered human cell lines. We mapped 39 mammalian ORs against 181 odorants and identified 79 interactions that have not been reported to our knowledge, including ligands for 15 previously orphaned receptors. This multiplexed receptor assay allows the cost-effective mapping of large chemical libraries to receptor repertoires at scale.

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Published In

Cell Syst

DOI

EISSN

2405-4720

Publication Date

March 27, 2019

Volume

8

Issue

3

Start / End Page

254 / 260.e6

Location

United States

Related Subject Headings

  • Smell
  • Signal Transduction
  • Sequence Analysis, RNA
  • Receptors, Odorant
  • Odorants
  • Mammals
  • Ligands
  • Humans
  • Gene Expression Profiling
  • Cell Line
 

Citation

APA
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ICMJE
MLA
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Jones, E. M., Jajoo, R., Cancilla, D., Lubock, N. B., Wang, J., Satyadi, M., … Kosuri, S. (2019). A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing. Cell Syst, 8(3), 254-260.e6. https://doi.org/10.1016/j.cels.2019.02.009
Jones, Eric M., Rishi Jajoo, Daniel Cancilla, Nathan B. Lubock, Jeffrey Wang, Megan Satyadi, Rockie Chong, et al. “A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing.Cell Syst 8, no. 3 (March 27, 2019): 254-260.e6. https://doi.org/10.1016/j.cels.2019.02.009.
Jones EM, Jajoo R, Cancilla D, Lubock NB, Wang J, Satyadi M, et al. A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing. Cell Syst. 2019 Mar 27;8(3):254-260.e6.
Jones, Eric M., et al. “A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing.Cell Syst, vol. 8, no. 3, Mar. 2019, pp. 254-260.e6. Pubmed, doi:10.1016/j.cels.2019.02.009.
Jones EM, Jajoo R, Cancilla D, Lubock NB, Wang J, Satyadi M, Chong R, de March C, Bloom JS, Matsunami H, Kosuri S. A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing. Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Published In

Cell Syst

DOI

EISSN

2405-4720

Publication Date

March 27, 2019

Volume

8

Issue

3

Start / End Page

254 / 260.e6

Location

United States

Related Subject Headings

  • Smell
  • Signal Transduction
  • Sequence Analysis, RNA
  • Receptors, Odorant
  • Odorants
  • Mammals
  • Ligands
  • Humans
  • Gene Expression Profiling
  • Cell Line