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Autoreactivity profiles of influenza hemagglutinin broadly neutralizing antibodies.

Publication ,  Journal Article
Bajic, G; van der Poel, CE; Kuraoka, M; Schmidt, AG; Carroll, MC; Kelsoe, G; Harrison, SC
Published in: Sci Rep
March 5, 2019

Epitope-focused approaches for selective clonal induction of broadly neutralizing antibodies (bnAbs) inform most current vaccine strategies for influenza virus and other rapidly evolving pathogens. The two conserved epitopes on the influenza hemagglutinin (HA) - the "stem" and the receptor-binding site (RBS) on the "head" - are the focus of the current "universal" influenza vaccine development efforts. Because stem-directed serum bnAbs are much less abundant than head-directed ones, we hypothesized that the HA stem bnAbs may be autoreactive and thus eliminated through the mechanisms of self-tolerance. We compared autoreactivity profiles of a set of stem and head-directed bnAbs. Most of the stem bnAbs we examined bound autoantigens; several showed staining of HEp-2 cells. A smaller proportion of the head-directed bnAbs were polyreactive. Gene usage did not correlate with autoreactivity. We suggest that complex foreign antigens may often have surface patches resembling some host epitope; our results indicate that HA stem epitopes resemble a host epitope more frequently than does the RBS.

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

March 5, 2019

Volume

9

Issue

1

Start / End Page

3492

Location

England

Related Subject Headings

  • Protein Array Analysis
  • Influenza Vaccines
  • Influenza A virus
  • Humans
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Epitopes
  • Cell Line
  • Broadly Neutralizing Antibodies
  • Autoantigens
  • Antigen-Antibody Reactions
 

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Bajic, G., van der Poel, C. E., Kuraoka, M., Schmidt, A. G., Carroll, M. C., Kelsoe, G., & Harrison, S. C. (2019). Autoreactivity profiles of influenza hemagglutinin broadly neutralizing antibodies. Sci Rep, 9(1), 3492. https://doi.org/10.1038/s41598-019-40175-8
Bajic, Goran, Cees E. van der Poel, Masayuki Kuraoka, Aaron G. Schmidt, Michael C. Carroll, Garnett Kelsoe, and Stephen C. Harrison. “Autoreactivity profiles of influenza hemagglutinin broadly neutralizing antibodies.Sci Rep 9, no. 1 (March 5, 2019): 3492. https://doi.org/10.1038/s41598-019-40175-8.
Bajic G, van der Poel CE, Kuraoka M, Schmidt AG, Carroll MC, Kelsoe G, et al. Autoreactivity profiles of influenza hemagglutinin broadly neutralizing antibodies. Sci Rep. 2019 Mar 5;9(1):3492.
Bajic, Goran, et al. “Autoreactivity profiles of influenza hemagglutinin broadly neutralizing antibodies.Sci Rep, vol. 9, no. 1, Mar. 2019, p. 3492. Pubmed, doi:10.1038/s41598-019-40175-8.
Bajic G, van der Poel CE, Kuraoka M, Schmidt AG, Carroll MC, Kelsoe G, Harrison SC. Autoreactivity profiles of influenza hemagglutinin broadly neutralizing antibodies. Sci Rep. 2019 Mar 5;9(1):3492.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

March 5, 2019

Volume

9

Issue

1

Start / End Page

3492

Location

England

Related Subject Headings

  • Protein Array Analysis
  • Influenza Vaccines
  • Influenza A virus
  • Humans
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Epitopes
  • Cell Line
  • Broadly Neutralizing Antibodies
  • Autoantigens
  • Antigen-Antibody Reactions