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Antibodies to a Conserved Influenza Head Interface Epitope Protect by an IgG Subtype-Dependent Mechanism.

Publication ,  Journal Article
Watanabe, A; McCarthy, KR; Kuraoka, M; Schmidt, AG; Adachi, Y; Onodera, T; Tonouchi, K; Caradonna, TM; Bajic, G; Song, S; McGee, CE; Feng, F ...
Published in: Cell
May 16, 2019

Vaccines to generate durable humoral immunity against antigenically evolving pathogens such as the influenza virus must elicit antibodies that recognize conserved epitopes. Analysis of single memory B cells from immunized human donors has led us to characterize a previously unrecognized epitope of influenza hemagglutinin (HA) that is immunogenic in humans and conserved among influenza subtypes. Structures show that an unrelated antibody from a participant in an experimental infection protocol recognized the epitope as well. IgGs specific for this antigenic determinant do not block viral infection in vitro, but passive administration to mice affords robust IgG subtype-dependent protection against influenza infection. The epitope, occluded in the pre-fusion form of HA, is at the contact surface between HA head domains; reversible molecular "breathing" of the HA trimer can expose the interface to antibody and B cells. Antigens that present this broadly immunogenic HA epitope may be good candidates for inclusion in "universal" flu vaccines.

Duke Scholars

Published In

Cell

DOI

EISSN

1097-4172

Publication Date

May 16, 2019

Volume

177

Issue

5

Start / End Page

1124 / 1135.e16

Location

United States

Related Subject Headings

  • Orthomyxoviridae Infections
  • Middle Aged
  • Mice
  • Male
  • Madin Darby Canine Kidney Cells
  • Influenza Vaccines
  • Influenza A virus
  • Immunoglobulin G
  • Humans
  • Hemagglutinin Glycoproteins, Influenza Virus
 

Citation

APA
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ICMJE
MLA
NLM
Watanabe, A., McCarthy, K. R., Kuraoka, M., Schmidt, A. G., Adachi, Y., Onodera, T., … Kelsoe, G. (2019). Antibodies to a Conserved Influenza Head Interface Epitope Protect by an IgG Subtype-Dependent Mechanism. Cell, 177(5), 1124-1135.e16. https://doi.org/10.1016/j.cell.2019.03.048
Journal cover image

Published In

Cell

DOI

EISSN

1097-4172

Publication Date

May 16, 2019

Volume

177

Issue

5

Start / End Page

1124 / 1135.e16

Location

United States

Related Subject Headings

  • Orthomyxoviridae Infections
  • Middle Aged
  • Mice
  • Male
  • Madin Darby Canine Kidney Cells
  • Influenza Vaccines
  • Influenza A virus
  • Immunoglobulin G
  • Humans
  • Hemagglutinin Glycoproteins, Influenza Virus