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Microparticles in the blood of patients with SLE: Size, content of mitochondria and role in circulating immune complexes.

Publication ,  Journal Article
Mobarrez, F; Fuzzi, E; Gunnarsson, I; Larsson, A; Eketjäll, S; Pisetsky, DS; Svenungsson, E
Published in: J Autoimmun
August 2019

OBJECTIVE: Microparticles (MPs) are small extracellular vesicles released from apoptotic or activated cells through a blebbing process. MPs express surface molecules from their parental cells and they bind IgG to form circulating immune complexes (MP-ICs) in patients with systemic lupus erythematosus (SLE). Through investigation of MP size, IgG expression, content of nucleic acids and mitochondrial molecules, we hypothesized that unrecognized particle populations can be identified in SLE. METHODS: We investigated 327 well-characterized SLE patients and 304 controls divided into two sets (280/280 and 47/24). We measured MPs by flow cytometry using a gating strategy to encompass small (0.2-0.7 μm) and large (0.7-3.0 μm) MPs. Nucleic acids were labeled with SYTO 13 and mitochondria with MitoTracker. Expression of mitochondria markers TOM-20 and Hexokinase 1 and the presence of IgG was investigated. RESULTS: MPs staining with SYTO 13 were more frequent in 280 SLE patients compared to 280 controls. In 47 SLE patients, levels of large MPs were elevated compared to 24 controls. The majority of large MPs contained mitochondria (mitoMPs). The number of mitoMPs associated positively with high disease activity, anti-dsDNA antibodies and pro-inflammatory cytokines. Patients with active lupus nephritis had higher levels of mitoMPs and IgG-positive mitoMPs. CONCLUSION: Blood of patients with SLE contain a previously unrecognized population of circulating large MPs with bound IgG and mitochondrial proteins. Levels of these particles are related to several measures of active SLE, suggesting that these structures may have a role in disease pathogenesis.

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Published In

J Autoimmun

DOI

EISSN

1095-9157

Publication Date

August 2019

Volume

102

Start / End Page

142 / 149

Location

England

Related Subject Headings

  • Mitochondrial Proteins
  • Mitochondria
  • Male
  • Lupus Erythematosus, Systemic
  • Immunology
  • Immunoglobulin G
  • Humans
  • Female
  • Cytokines
  • Cell-Free Nucleic Acids
 

Citation

APA
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ICMJE
MLA
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Mobarrez, F., Fuzzi, E., Gunnarsson, I., Larsson, A., Eketjäll, S., Pisetsky, D. S., & Svenungsson, E. (2019). Microparticles in the blood of patients with SLE: Size, content of mitochondria and role in circulating immune complexes. J Autoimmun, 102, 142–149. https://doi.org/10.1016/j.jaut.2019.05.003
Mobarrez, Fariborz, Enrico Fuzzi, Iva Gunnarsson, Anders Larsson, Susanna Eketjäll, David S. Pisetsky, and Elisabet Svenungsson. “Microparticles in the blood of patients with SLE: Size, content of mitochondria and role in circulating immune complexes.J Autoimmun 102 (August 2019): 142–49. https://doi.org/10.1016/j.jaut.2019.05.003.
Mobarrez F, Fuzzi E, Gunnarsson I, Larsson A, Eketjäll S, Pisetsky DS, et al. Microparticles in the blood of patients with SLE: Size, content of mitochondria and role in circulating immune complexes. J Autoimmun. 2019 Aug;102:142–9.
Mobarrez, Fariborz, et al. “Microparticles in the blood of patients with SLE: Size, content of mitochondria and role in circulating immune complexes.J Autoimmun, vol. 102, Aug. 2019, pp. 142–49. Pubmed, doi:10.1016/j.jaut.2019.05.003.
Mobarrez F, Fuzzi E, Gunnarsson I, Larsson A, Eketjäll S, Pisetsky DS, Svenungsson E. Microparticles in the blood of patients with SLE: Size, content of mitochondria and role in circulating immune complexes. J Autoimmun. 2019 Aug;102:142–149.
Journal cover image

Published In

J Autoimmun

DOI

EISSN

1095-9157

Publication Date

August 2019

Volume

102

Start / End Page

142 / 149

Location

England

Related Subject Headings

  • Mitochondrial Proteins
  • Mitochondria
  • Male
  • Lupus Erythematosus, Systemic
  • Immunology
  • Immunoglobulin G
  • Humans
  • Female
  • Cytokines
  • Cell-Free Nucleic Acids