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The next generation of CRISPR-Cas technologies and applications.

Publication ,  Journal Article
Pickar-Oliver, A; Gersbach, CA
Published in: Nature reviews. Molecular cell biology
August 2019

The prokaryote-derived CRISPR-Cas genome editing systems have transformed our ability to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells of diverse species. The ease of use and robustness of this technology have revolutionized genome editing for research ranging from fundamental science to translational medicine. Initial successes have inspired efforts to discover new systems for targeting and manipulating nucleic acids, including those from Cas9, Cas12, Cascade and Cas13 orthologues. Genome editing by CRISPR-Cas can utilize non-homologous end joining and homology-directed repair for DNA repair, as well as single-base editing enzymes. In addition to targeting DNA, CRISPR-Cas-based RNA-targeting tools are being developed for research, medicine and diagnostics. Nuclease-inactive and RNA-targeting Cas proteins have been fused to a plethora of effector proteins to regulate gene expression, epigenetic modifications and chromatin interactions. Collectively, the new advances are considerably improving our understanding of biological processes and are propelling CRISPR-Cas-based tools towards clinical use in gene and cell therapies.

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Published In

Nature reviews. Molecular cell biology

DOI

EISSN

1471-0080

ISSN

1471-0072

Publication Date

August 2019

Volume

20

Issue

8

Start / End Page

490 / 507

Related Subject Headings

  • RNA, Guide, CRISPR-Cas Systems
  • Humans
  • Genetic Therapy
  • Gene Editing
  • Endonucleases
  • Developmental Biology
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • CRISPR-Cas Systems
  • Animals
  • 42 Health sciences
 

Citation

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Pickar-Oliver, A., & Gersbach, C. A. (2019). The next generation of CRISPR-Cas technologies and applications. Nature Reviews. Molecular Cell Biology, 20(8), 490–507. https://doi.org/10.1038/s41580-019-0131-5
Pickar-Oliver, Adrian, and Charles A. Gersbach. “The next generation of CRISPR-Cas technologies and applications.Nature Reviews. Molecular Cell Biology 20, no. 8 (August 2019): 490–507. https://doi.org/10.1038/s41580-019-0131-5.
Pickar-Oliver A, Gersbach CA. The next generation of CRISPR-Cas technologies and applications. Nature reviews Molecular cell biology. 2019 Aug;20(8):490–507.
Pickar-Oliver, Adrian, and Charles A. Gersbach. “The next generation of CRISPR-Cas technologies and applications.Nature Reviews. Molecular Cell Biology, vol. 20, no. 8, Aug. 2019, pp. 490–507. Epmc, doi:10.1038/s41580-019-0131-5.
Pickar-Oliver A, Gersbach CA. The next generation of CRISPR-Cas technologies and applications. Nature reviews Molecular cell biology. 2019 Aug;20(8):490–507.

Published In

Nature reviews. Molecular cell biology

DOI

EISSN

1471-0080

ISSN

1471-0072

Publication Date

August 2019

Volume

20

Issue

8

Start / End Page

490 / 507

Related Subject Headings

  • RNA, Guide, CRISPR-Cas Systems
  • Humans
  • Genetic Therapy
  • Gene Editing
  • Endonucleases
  • Developmental Biology
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • CRISPR-Cas Systems
  • Animals
  • 42 Health sciences