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Inducible Fibril Formation of Silk-Elastin Diblocks.

Publication ,  Journal Article
Willems, L; Roberts, S; Weitzhandler, I; Chilkoti, A; Mastrobattista, E; van der Oost, J; de Vries, R
Published in: ACS omega
May 2019

Silk-elastin block copolymers have such physical and biological properties that make them attractive biomaterials for applications ranging from tissue regeneration to drug delivery. Silk-elastin block copolymers that only assemble into fibrils at high concentrations can be used for a template-induced fibril assembly. This can be achieved by additionally including template-binding blocks that promote high local concentrations of polymers on the template, leading to a template-induced fibril assembly. We hypothesize that template-inducible silk-fibril formation, and hence high critical concentrations for fibril formation, requires careful tuning of the block lengths, to be close to a critical set of block lengths that separates fibril forming from nonfibril forming polymer architectures. Therefore, we explore herein the impact of tuning block lengths for silk-elastin diblock polypeptides on fibril formation. For silk-elastin diblocks ESm -SQn , in which the elastin pentamer repeat is ES = GSGVP and the crystallizable silk octamer repeat is SQ = GAGAGAGQ, we find that no fibril formation occurs for n = 6 but that the n = 10 and 14 diblocks do show concentration-dependent fibril formation. For n = 14 diblocks, no effect is observed of the length m (with m = 40, 60, 80) of the amorphous block on the lengths of the fibrils. In contrast, for the n = 10 diblocks that are closest to the critical boundary for fibril formation, we find that long amorphous blocks (m = 80) oppose the growth of fibrils at low concentrations, making them suitable for engineering template-inducible fibril formation.

Duke Scholars

Published In

ACS omega

DOI

EISSN

2470-1343

ISSN

2470-1343

Publication Date

May 2019

Volume

4

Issue

5

Start / End Page

9135 / 9143

Related Subject Headings

  • 4004 Chemical engineering
  • 3406 Physical chemistry
  • 3403 Macromolecular and materials chemistry
  • 0912 Materials Engineering
  • 0904 Chemical Engineering
 

Citation

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ICMJE
MLA
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Willems, L., Roberts, S., Weitzhandler, I., Chilkoti, A., Mastrobattista, E., van der Oost, J., & de Vries, R. (2019). Inducible Fibril Formation of Silk-Elastin Diblocks. ACS Omega, 4(5), 9135–9143. https://doi.org/10.1021/acsomega.9b01025
Willems, Lione, Stefan Roberts, Isaac Weitzhandler, Ashutosh Chilkoti, Enrico Mastrobattista, John van der Oost, and Renko de Vries. “Inducible Fibril Formation of Silk-Elastin Diblocks.ACS Omega 4, no. 5 (May 2019): 9135–43. https://doi.org/10.1021/acsomega.9b01025.
Willems L, Roberts S, Weitzhandler I, Chilkoti A, Mastrobattista E, van der Oost J, et al. Inducible Fibril Formation of Silk-Elastin Diblocks. ACS omega. 2019 May;4(5):9135–43.
Willems, Lione, et al. “Inducible Fibril Formation of Silk-Elastin Diblocks.ACS Omega, vol. 4, no. 5, May 2019, pp. 9135–43. Epmc, doi:10.1021/acsomega.9b01025.
Willems L, Roberts S, Weitzhandler I, Chilkoti A, Mastrobattista E, van der Oost J, de Vries R. Inducible Fibril Formation of Silk-Elastin Diblocks. ACS omega. 2019 May;4(5):9135–9143.

Published In

ACS omega

DOI

EISSN

2470-1343

ISSN

2470-1343

Publication Date

May 2019

Volume

4

Issue

5

Start / End Page

9135 / 9143

Related Subject Headings

  • 4004 Chemical engineering
  • 3406 Physical chemistry
  • 3403 Macromolecular and materials chemistry
  • 0912 Materials Engineering
  • 0904 Chemical Engineering