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Systematic analysis of F-box proteins reveals a new branch of the yeast mating pathway.

Publication ,  Journal Article
Rangarajan, N; Gordy, CL; Askew, L; Bevill, SM; Elston, TC; Errede, B; Hurst, JH; Kelley, JB; Sheetz, JB; Suzuki, SK; Valentin, NH; Young, E ...
Published in: J Biol Chem
October 4, 2019

The mating pathway in yeast Saccharomyces cerevisiae has long been used to reveal new mechanisms of signal transduction. The pathway comprises a pheromone receptor, a heterotrimeric G protein, and intracellular effectors of morphogenesis and transcription. Polarized cell growth, in the direction of a potential mating partner, is accomplished by the G-protein βγ subunits and the small G-protein Cdc42. Transcription induction, needed for cell-cell fusion, is mediated by Gβγ and the mitogen-activated protein kinase (MAPK) scaffold protein Ste5. A potential third pathway is initiated by the G-protein α subunit Gpa1. Gpa1 signaling was shown previously to involve the F-box adaptor protein Dia2 and an endosomal effector protein, the phosphatidylinositol 3-kinase Vps34. Vps34 is also required for proper vacuolar sorting and autophagy. Here, using a panel of reporter assays, we demonstrate that mating pheromone stimulates vacuolar targeting of a cytoplasmic reporter protein and that this process depends on Vps34. Through a systematic analysis of F-box deletion mutants, we show that Dia2 is required to sustain pheromone-induced vacuolar targeting. We also found that other F-box proteins selectively regulate morphogenesis (Ydr306, renamed Pfu1) and transcription (Ucc1). These findings point to the existence of a new and distinct branch of the pheromone-signaling pathway, one that likely leads to vacuolar engulfment of cytoplasmic proteins and recycling of cellular contents in preparation for mating.

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Published In

J Biol Chem

DOI

EISSN

1083-351X

Publication Date

October 4, 2019

Volume

294

Issue

40

Start / End Page

14717 / 14731

Location

United States

Related Subject Headings

  • cdc42 GTP-Binding Protein
  • Vacuoles
  • Transcription, Genetic
  • Signal Transduction
  • Sequence Deletion
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Pheromones
  • Morphogenesis
  • Genes, Mating Type, Fungal
 

Citation

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Rangarajan, N., Gordy, C. L., Askew, L., Bevill, S. M., Elston, T. C., Errede, B., … Dohlman, H. G. (2019). Systematic analysis of F-box proteins reveals a new branch of the yeast mating pathway. J Biol Chem, 294(40), 14717–14731. https://doi.org/10.1074/jbc.RA119.010063
Rangarajan, Nambirajan, Claire L. Gordy, Lauren Askew, Samantha M. Bevill, Timothy C. Elston, Beverly Errede, Jillian H. Hurst, et al. “Systematic analysis of F-box proteins reveals a new branch of the yeast mating pathway.J Biol Chem 294, no. 40 (October 4, 2019): 14717–31. https://doi.org/10.1074/jbc.RA119.010063.
Rangarajan N, Gordy CL, Askew L, Bevill SM, Elston TC, Errede B, et al. Systematic analysis of F-box proteins reveals a new branch of the yeast mating pathway. J Biol Chem. 2019 Oct 4;294(40):14717–31.
Rangarajan, Nambirajan, et al. “Systematic analysis of F-box proteins reveals a new branch of the yeast mating pathway.J Biol Chem, vol. 294, no. 40, Oct. 2019, pp. 14717–31. Pubmed, doi:10.1074/jbc.RA119.010063.
Rangarajan N, Gordy CL, Askew L, Bevill SM, Elston TC, Errede B, Hurst JH, Kelley JB, Sheetz JB, Suzuki SK, Valentin NH, Young E, Dohlman HG. Systematic analysis of F-box proteins reveals a new branch of the yeast mating pathway. J Biol Chem. 2019 Oct 4;294(40):14717–14731.

Published In

J Biol Chem

DOI

EISSN

1083-351X

Publication Date

October 4, 2019

Volume

294

Issue

40

Start / End Page

14717 / 14731

Location

United States

Related Subject Headings

  • cdc42 GTP-Binding Protein
  • Vacuoles
  • Transcription, Genetic
  • Signal Transduction
  • Sequence Deletion
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Pheromones
  • Morphogenesis
  • Genes, Mating Type, Fungal