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Site-Specific K63 Ubiquitinomics Provides Insights into Translation Regulation under Stress.

Publication ,  Journal Article
Back, S; Gorman, AW; Vogel, C; Silva, GM
Published in: Journal of proteome research
January 2019

During oxidative stress, K63-linked polyubiquitin chains modify a variety of proteins including ribosomes. Knowledge of the precise sites of K63 ubiquitin is key to understand its function during the response to stress. To identify the sites of K63 ubiquitin, we developed a new mass spectrometry based method that quantified >1100 K63 ubiquitination sites in yeast that responded to oxidative stress induced by H2O2. We determined that under stress, K63 ubiquitin-modified proteins were involved in several cellular functions including ion transport, protein trafficking, and translation. The most abundant ubiquitin sites localized to the head of the 40S subunit of the ribosome, modified assembled polysomes, and affected the binding of translation factors. The results suggested a new pathway of post-initiation control of translation during oxidative stress and illustrated the importance of high-resolution mapping of noncanonical ubiquitination events.

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Published In

Journal of proteome research

DOI

EISSN

1535-3907

ISSN

1535-3893

Publication Date

January 2019

Volume

18

Issue

1

Start / End Page

309 / 318

Related Subject Headings

  • Yeasts
  • Ubiquitination
  • Ubiquitin
  • Ribosomes
  • Proteomics
  • Protein Processing, Post-Translational
  • Protein Biosynthesis
  • Oxidative Stress
  • Hydrogen Peroxide
  • Fungal Proteins
 

Citation

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Back, S., Gorman, A. W., Vogel, C., & Silva, G. M. (2019). Site-Specific K63 Ubiquitinomics Provides Insights into Translation Regulation under Stress. Journal of Proteome Research, 18(1), 309–318. https://doi.org/10.1021/acs.jproteome.8b00623
Back, Songhee, Andrew W. Gorman, Christine Vogel, and Gustavo M. Silva. “Site-Specific K63 Ubiquitinomics Provides Insights into Translation Regulation under Stress.Journal of Proteome Research 18, no. 1 (January 2019): 309–18. https://doi.org/10.1021/acs.jproteome.8b00623.
Back S, Gorman AW, Vogel C, Silva GM. Site-Specific K63 Ubiquitinomics Provides Insights into Translation Regulation under Stress. Journal of proteome research. 2019 Jan;18(1):309–18.
Back, Songhee, et al. “Site-Specific K63 Ubiquitinomics Provides Insights into Translation Regulation under Stress.Journal of Proteome Research, vol. 18, no. 1, Jan. 2019, pp. 309–18. Epmc, doi:10.1021/acs.jproteome.8b00623.
Back S, Gorman AW, Vogel C, Silva GM. Site-Specific K63 Ubiquitinomics Provides Insights into Translation Regulation under Stress. Journal of proteome research. 2019 Jan;18(1):309–318.
Journal cover image

Published In

Journal of proteome research

DOI

EISSN

1535-3907

ISSN

1535-3893

Publication Date

January 2019

Volume

18

Issue

1

Start / End Page

309 / 318

Related Subject Headings

  • Yeasts
  • Ubiquitination
  • Ubiquitin
  • Ribosomes
  • Proteomics
  • Protein Processing, Post-Translational
  • Protein Biosynthesis
  • Oxidative Stress
  • Hydrogen Peroxide
  • Fungal Proteins