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Exploiting codon usage identifies RpS21 as an in vivo signal strength-dependent Ras/MAPK regulator

Publication ,  Journal Article
Sawyer, J; Kabiri, Z; Montague, R; Paramore, S; Cohen, E; Zaribafzadeh, H; Counter, C; Fox, D
May 26, 2019

ABSTRACT Signal transduction pathways are intricately fine-tuned to accomplish diverse biological processes. An example is the conserved Ras/mitogen-activated-protein-kinase (MAPK) pathway, which exhibits context-dependent signaling output dynamics and regulation. Here, by altering codon usage as a novel platform to control signaling output, we screened the Drosophila genome for modifiers specific to either weak or strong Ras-driven eye phenotypes. We mapped the underlying gene from one modifier to the ribosomal gene RpS21 . RpS21 preferentially influences weak Ras/MAPK signaling outputs, and negatively regulates Ras/MAPK in multiple cell/tissue and signaling settings. In turn, MAPK signaling may regulate its own negative feedback by promoting RpS21 expression. These data show that codon usage manipulation can identify output-specific signaling regulators, and identify RpS21 as an in vivo Ras/MAPK phenotypic regulator.

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May 26, 2019
 

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Sawyer, J., Kabiri, Z., Montague, R., Paramore, S., Cohen, E., Zaribafzadeh, H., … Fox, D. (2019). Exploiting codon usage identifies RpS21 as an in vivo signal strength-dependent Ras/MAPK regulator. https://doi.org/10.1101/650630
Sawyer, Jessica, Zahra Kabiri, Ruth Montague, Sarah Paramore, Erez Cohen, Hamed Zaribafzadeh, Christopher Counter, and Donald Fox. “Exploiting codon usage identifies RpS21 as an in vivo signal strength-dependent Ras/MAPK regulator,” May 26, 2019. https://doi.org/10.1101/650630.
Sawyer J, Kabiri Z, Montague R, Paramore S, Cohen E, Zaribafzadeh H, et al. Exploiting codon usage identifies RpS21 as an in vivo signal strength-dependent Ras/MAPK regulator. 2019 May 26;
Sawyer J, Kabiri Z, Montague R, Paramore S, Cohen E, Zaribafzadeh H, Counter C, Fox D. Exploiting codon usage identifies RpS21 as an in vivo signal strength-dependent Ras/MAPK regulator. 2019 May 26;

DOI

Publication Date

May 26, 2019