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α-Integrins dictate distinct modes of type IV collagen recruitment to basement membranes.

Publication ,  Journal Article
Jayadev, R; Chi, Q; Keeley, DP; Hastie, EL; Kelley, LC; Sherwood, DR
Published in: The Journal of cell biology
September 2019

Basement membranes (BMs) are cell-associated extracellular matrices that support tissue integrity, signaling, and barrier properties. Type IV collagen is critical for BM function, yet how it is directed into BMs in vivo is unclear. Through live-cell imaging of endogenous localization, conditional knockdown, and misexpression experiments, we uncovered distinct mechanisms of integrin-mediated collagen recruitment to Caenorhabditis elegans postembryonic gonadal and pharyngeal BMs. The putative laminin-binding αINA-1/βPAT-3 integrin was selectively activated in the gonad and recruited laminin, which directed moderate collagen incorporation. In contrast, the putative Arg-Gly-Asp (RGD)-binding αPAT-2/βPAT-3 integrin was activated in the pharynx and recruited high levels of collagen in an apparently laminin-independent manner. Through an RNAi screen, we further identified the small GTPase RAP-3 (Rap1) as a pharyngeal-specific PAT-2/PAT-3 activator that modulates collagen levels. Together, these studies demonstrate that tissues can use distinct mechanisms to direct collagen incorporation into BMs to precisely control collagen levels and construct diverse BMs.

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Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

September 2019

Volume

218

Issue

9

Start / End Page

3098 / 3116

Related Subject Headings

  • Integrin beta Chains
  • Developmental Biology
  • Collagen Type IV
  • Caenorhabditis elegans Proteins
  • Caenorhabditis elegans
  • Basement Membrane
  • Animals
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 11 Medical and Health Sciences
 

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Jayadev, R., Chi, Q., Keeley, D. P., Hastie, E. L., Kelley, L. C., & Sherwood, D. R. (2019). α-Integrins dictate distinct modes of type IV collagen recruitment to basement membranes. The Journal of Cell Biology, 218(9), 3098–3116. https://doi.org/10.1083/jcb.201903124
Jayadev, Ranjay, Qiuyi Chi, Daniel P. Keeley, Eric L. Hastie, Laura C. Kelley, and David R. Sherwood. “α-Integrins dictate distinct modes of type IV collagen recruitment to basement membranes.The Journal of Cell Biology 218, no. 9 (September 2019): 3098–3116. https://doi.org/10.1083/jcb.201903124.
Jayadev R, Chi Q, Keeley DP, Hastie EL, Kelley LC, Sherwood DR. α-Integrins dictate distinct modes of type IV collagen recruitment to basement membranes. The Journal of cell biology. 2019 Sep;218(9):3098–116.
Jayadev, Ranjay, et al. “α-Integrins dictate distinct modes of type IV collagen recruitment to basement membranes.The Journal of Cell Biology, vol. 218, no. 9, Sept. 2019, pp. 3098–116. Epmc, doi:10.1083/jcb.201903124.
Jayadev R, Chi Q, Keeley DP, Hastie EL, Kelley LC, Sherwood DR. α-Integrins dictate distinct modes of type IV collagen recruitment to basement membranes. The Journal of cell biology. 2019 Sep;218(9):3098–3116.

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

September 2019

Volume

218

Issue

9

Start / End Page

3098 / 3116

Related Subject Headings

  • Integrin beta Chains
  • Developmental Biology
  • Collagen Type IV
  • Caenorhabditis elegans Proteins
  • Caenorhabditis elegans
  • Basement Membrane
  • Animals
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 11 Medical and Health Sciences