Skip to main content
Journal cover image

Transcriptomic profiles of aging in purified human immune cells.

Publication ,  Journal Article
Reynolds, LM; Ding, J; Taylor, JR; Lohman, K; Soranzo, N; de la Fuente, A; Liu, TF; Johnson, C; Barr, RG; Register, TC; Donohue, KM; Talor, MV ...
Published in: BMC Genomics
April 22, 2015

BACKGROUND: Transcriptomic studies hold great potential towards understanding the human aging process. Previous transcriptomic studies have identified many genes with age-associated expression levels; however, small samples sizes and mixed cell types often make these results difficult to interpret. RESULTS: Using transcriptomic profiles in CD14+ monocytes from 1,264 participants of the Multi-Ethnic Study of Atherosclerosis (aged 55-94 years), we identified 2,704 genes differentially expressed with chronological age (false discovery rate, FDR ≤ 0.001). We further identified six networks of co-expressed genes that included prominent genes from three pathways: protein synthesis (particularly mitochondrial ribosomal genes), oxidative phosphorylation, and autophagy, with expression patterns suggesting these pathways decline with age. Expression of several chromatin remodeler and transcriptional modifier genes strongly correlated with expression of oxidative phosphorylation and ribosomal protein synthesis genes. 17% of genes with age-associated expression harbored CpG sites whose degree of methylation significantly mediated the relationship between age and gene expression (p < 0.05). Lastly, 15 genes with age-associated expression were also associated (FDR ≤ 0.01) with pulse pressure independent of chronological age. Comparing transcriptomic profiles of CD14+ monocytes to CD4+ T cells from a subset (n = 423) of the population, we identified 30 age-associated (FDR < 0.01) genes in common, while larger sets of differentially expressed genes were unique to either T cells (188 genes) or monocytes (383 genes). At the pathway level, a decline in ribosomal protein synthesis machinery gene expression with age was detectable in both cell types. CONCLUSIONS: An overall decline in expression of ribosomal protein synthesis genes with age was detected in CD14+ monocytes and CD4+ T cells, demonstrating that some patterns of aging are likely shared between different cell types. Our findings also support cell-specific effects of age on gene expression, illustrating the importance of using purified cell samples for future transcriptomic studies. Longitudinal work is required to establish the relationship between identified age-associated genes/pathways and aging-related diseases.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

BMC Genomics

DOI

EISSN

1471-2164

Publication Date

April 22, 2015

Volume

16

Issue

1

Start / End Page

333

Location

England

Related Subject Headings

  • Transcriptome
  • T-Lymphocytes
  • Ribosomes
  • Protein Biosynthesis
  • Oxidative Phosphorylation
  • Monocytes
  • Middle Aged
  • Male
  • Lipopolysaccharide Receptors
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Reynolds, L. M., Ding, J., Taylor, J. R., Lohman, K., Soranzo, N., de la Fuente, A., … Liu, Y. (2015). Transcriptomic profiles of aging in purified human immune cells. BMC Genomics, 16(1), 333. https://doi.org/10.1186/s12864-015-1522-4
Reynolds, Lindsay M., Jingzhong Ding, Jackson R. Taylor, Kurt Lohman, Nicola Soranzo, Alberto de la Fuente, Tie Fu Liu, et al. “Transcriptomic profiles of aging in purified human immune cells.BMC Genomics 16, no. 1 (April 22, 2015): 333. https://doi.org/10.1186/s12864-015-1522-4.
Reynolds LM, Ding J, Taylor JR, Lohman K, Soranzo N, de la Fuente A, et al. Transcriptomic profiles of aging in purified human immune cells. BMC Genomics. 2015 Apr 22;16(1):333.
Reynolds, Lindsay M., et al. “Transcriptomic profiles of aging in purified human immune cells.BMC Genomics, vol. 16, no. 1, Apr. 2015, p. 333. Pubmed, doi:10.1186/s12864-015-1522-4.
Reynolds LM, Ding J, Taylor JR, Lohman K, Soranzo N, de la Fuente A, Liu TF, Johnson C, Barr RG, Register TC, Donohue KM, Talor MV, Cihakova D, Gu C, Divers J, Siscovick D, Burke G, Post W, Shea S, Jacobs DR, Hoeschele I, McCall CE, Kritchevsky SB, Herrington D, Tracy RP, Liu Y. Transcriptomic profiles of aging in purified human immune cells. BMC Genomics. 2015 Apr 22;16(1):333.
Journal cover image

Published In

BMC Genomics

DOI

EISSN

1471-2164

Publication Date

April 22, 2015

Volume

16

Issue

1

Start / End Page

333

Location

England

Related Subject Headings

  • Transcriptome
  • T-Lymphocytes
  • Ribosomes
  • Protein Biosynthesis
  • Oxidative Phosphorylation
  • Monocytes
  • Middle Aged
  • Male
  • Lipopolysaccharide Receptors
  • Humans