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OGP functionalized phenylalanine-based poly(ester urea) for enhancing osteoinductive potential of human mesenchymal stem cells.

Publication ,  Journal Article
Policastro, GM; Lin, F; Smith Callahan, LA; Esterle, A; Graham, M; Sloan Stakleff, K; Becker, ML
Published in: Biomacromolecules
April 2015

Amino acid-based poly(ester urea)s (PEU) are high modulus, resorbable polymers with many potential uses, including the surgical repair of bone defects. In vitro and in vivo studies have previously shown that phenylalanine-based PEUs have nontoxic hydrolytic byproducts and tunable degradation times. Phenylalanine PEUs (poly(1-PHE-6)) have been further modified by tethering osteogenic growth peptide (OGP) to tyrosine-based monomer subunits. These OGP-tethered PEUs have been fabricated into porous scaffolds and cultured in vitro to examine their effect on differentiation of human mesenchymal stem cells (hMSCs) toward the osteogenic lineage. The influence of tethered OGP on the hMSC proliferation and differentiation profile was measured using immunohistochemistry, biochemistry, and quantitative real time polymerase chain reaction (qRT-PCR). In vitro data indicated an enhanced expression of BSP by 130-160% for hMSCs on OGP-tethered scaffolds compared to controls. By 4 weeks, there was a significant drop (60-85% decrease) in BSP expression on OGP-functionalized scaffolds, which is characteristic of osteogenic differentiation. ALP and OSC expression was significantly enhanced for OGP-functionalized scaffolds by week 4, with values reaching 145% and 300% greater, respectively, compared to nonfunctionalized controls. In vivo subcutaneous implantation of poly(1-PHE-6) scaffolds revealed significant tissue-scaffold integration, as well as the promotion of both osteogenesis and angiogenesis.

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Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

April 2015

Volume

16

Issue

4

Start / End Page

1358 / 1371

Related Subject Headings

  • Urea
  • Tissue Scaffolds
  • Tissue Engineering
  • Rats, Sprague-Dawley
  • Rats
  • Polymers
  • Polyesters
  • Phenylalanine
  • Osteogenesis
  • Mesenchymal Stem Cells
 

Citation

APA
Chicago
ICMJE
MLA
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Policastro, G. M., Lin, F., Smith Callahan, L. A., Esterle, A., Graham, M., Sloan Stakleff, K., & Becker, M. L. (2015). OGP functionalized phenylalanine-based poly(ester urea) for enhancing osteoinductive potential of human mesenchymal stem cells. Biomacromolecules, 16(4), 1358–1371. https://doi.org/10.1021/acs.biomac.5b00153
Policastro, Gina M., Fei Lin, Laura A. Smith Callahan, Andrew Esterle, Matthew Graham, Kimberly Sloan Stakleff, and Matthew L. Becker. “OGP functionalized phenylalanine-based poly(ester urea) for enhancing osteoinductive potential of human mesenchymal stem cells.Biomacromolecules 16, no. 4 (April 2015): 1358–71. https://doi.org/10.1021/acs.biomac.5b00153.
Policastro GM, Lin F, Smith Callahan LA, Esterle A, Graham M, Sloan Stakleff K, et al. OGP functionalized phenylalanine-based poly(ester urea) for enhancing osteoinductive potential of human mesenchymal stem cells. Biomacromolecules. 2015 Apr;16(4):1358–71.
Policastro, Gina M., et al. “OGP functionalized phenylalanine-based poly(ester urea) for enhancing osteoinductive potential of human mesenchymal stem cells.Biomacromolecules, vol. 16, no. 4, Apr. 2015, pp. 1358–71. Epmc, doi:10.1021/acs.biomac.5b00153.
Policastro GM, Lin F, Smith Callahan LA, Esterle A, Graham M, Sloan Stakleff K, Becker ML. OGP functionalized phenylalanine-based poly(ester urea) for enhancing osteoinductive potential of human mesenchymal stem cells. Biomacromolecules. 2015 Apr;16(4):1358–1371.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

April 2015

Volume

16

Issue

4

Start / End Page

1358 / 1371

Related Subject Headings

  • Urea
  • Tissue Scaffolds
  • Tissue Engineering
  • Rats, Sprague-Dawley
  • Rats
  • Polymers
  • Polyesters
  • Phenylalanine
  • Osteogenesis
  • Mesenchymal Stem Cells