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Facile fabrication of "dual click" one- and two-dimensional orthogonal peptide concentration gradients.

Publication ,  Journal Article
Ma, Y; Zheng, J; Amond, EF; Stafford, CM; Becker, ML
Published in: Biomacromolecules
March 2013

Peptides, proteins, and extracellular matrix act synergistically to influence cellular function at the biotic-synthetic interface. However, identifying the individual and cooperative contributions of the various combinations and concentration regimes is extremely difficult. The confined channel deposition method we describe affords highly tunable orthogonal reactive concentration gradients that greatly expand the dynamic range, spatial control, and chemical versatility of the reactive silanes that can be controllably deposited. Using metal-free "dual click" immobilization chemistries, multiple peptides with a variety of functionality can be immobilized efficiently and reproducibly enabling optimal concentration profiling and the assessment of synergistic interactions.

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Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

March 2013

Volume

14

Issue

3

Start / End Page

665 / 671

Related Subject Headings

  • Ultraviolet Rays
  • Surface Properties
  • Silanes
  • Polymers
  • Photoelectron Spectroscopy
  • Peptides
  • Ozone
  • Immobilization
  • Glass
  • Cycloaddition Reaction
 

Citation

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Ma, Y., Zheng, J., Amond, E. F., Stafford, C. M., & Becker, M. L. (2013). Facile fabrication of "dual click" one- and two-dimensional orthogonal peptide concentration gradients. Biomacromolecules, 14(3), 665–671. https://doi.org/10.1021/bm301731h
Ma, Yanrui, Jukuan Zheng, Emily F. Amond, Christopher M. Stafford, and Matthew L. Becker. “Facile fabrication of "dual click" one- and two-dimensional orthogonal peptide concentration gradients.Biomacromolecules 14, no. 3 (March 2013): 665–71. https://doi.org/10.1021/bm301731h.
Ma Y, Zheng J, Amond EF, Stafford CM, Becker ML. Facile fabrication of "dual click" one- and two-dimensional orthogonal peptide concentration gradients. Biomacromolecules. 2013 Mar;14(3):665–71.
Ma, Yanrui, et al. “Facile fabrication of "dual click" one- and two-dimensional orthogonal peptide concentration gradients.Biomacromolecules, vol. 14, no. 3, Mar. 2013, pp. 665–71. Epmc, doi:10.1021/bm301731h.
Ma Y, Zheng J, Amond EF, Stafford CM, Becker ML. Facile fabrication of "dual click" one- and two-dimensional orthogonal peptide concentration gradients. Biomacromolecules. 2013 Mar;14(3):665–671.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

March 2013

Volume

14

Issue

3

Start / End Page

665 / 671

Related Subject Headings

  • Ultraviolet Rays
  • Surface Properties
  • Silanes
  • Polymers
  • Photoelectron Spectroscopy
  • Peptides
  • Ozone
  • Immobilization
  • Glass
  • Cycloaddition Reaction