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High-content imaging-based screening of microenvironment-induced changes to stem cells.

Publication ,  Journal Article
Vega, SL; Liu, E; Patel, PJ; Kulesa, AB; Carlson, AL; Ma, Y; Becker, ML; Moghe, PV
Published in: Journal of biomolecular screening
October 2012

Effective screening methodologies for cells are challenged by the divergent and heterogeneous nature of phenotypes inherent to stem cell cultures, particularly on engineered biomaterial surfaces. In this study, we showcase a high-content, confocal imaging-based methodology to parse single-cell phenotypes by quantifying organizational signatures of specific subcellular reporter proteins and applied this profiling approach to three human stem cell types (embryonic-human embryonic stem cell [hESC], induced pluripotent-induced pluripotent stem cell [iPSC], and mesenchymal-human mesenchymal stem cell [hMSC]). We demonstrate that this method could distinguish self-renewing subpopulations of hESCs and iPSCs from heterogeneous populations. This technique can also provide insights into how incremental changes in biomaterial properties, both physiochemical and mechanical, influence stem cell fates by parsing the organization of stem cell proteins. For example, hMSCs cultured on polymeric films with varying degrees of poly(ethylene glycol) to modulate osteogenic differentiation were parsed using high-content organization of the cytoskeletal protein F-actin. In addition, hMSCs cultured on a self-assembled monolayer platform featuring compositional gradients were screened and descriptors obtained to correlate substrate variations with adipogenic lineage commitment. Taken together, high-content imaging of structurally sensitive proteins can be used as a tool to identify stem cell phenotypes at the single-cell level across a diverse range of culture conditions and microenvironments.

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Published In

Journal of biomolecular screening

DOI

EISSN

1552-454X

ISSN

1087-0571

Publication Date

October 2012

Volume

17

Issue

9

Start / End Page

1151 / 1162

Related Subject Headings

  • Tissue Scaffolds
  • SOXB1 Transcription Factors
  • Principal Component Analysis
  • Polyethylene Glycols
  • Osteogenesis
  • Nuclear Matrix-Associated Proteins
  • Microscopy, Confocal
  • Mesenchymal Stem Cells
  • Medicinal & Biomolecular Chemistry
  • Induced Pluripotent Stem Cells
 

Citation

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ICMJE
MLA
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Vega, S. L., Liu, E., Patel, P. J., Kulesa, A. B., Carlson, A. L., Ma, Y., … Moghe, P. V. (2012). High-content imaging-based screening of microenvironment-induced changes to stem cells. Journal of Biomolecular Screening, 17(9), 1151–1162. https://doi.org/10.1177/1087057112453853
Vega, Sebastián L., Er Liu, Parth J. Patel, Anthony B. Kulesa, Aaron L. Carlson, Yanrui Ma, Matthew L. Becker, and Prabhas V. Moghe. “High-content imaging-based screening of microenvironment-induced changes to stem cells.Journal of Biomolecular Screening 17, no. 9 (October 2012): 1151–62. https://doi.org/10.1177/1087057112453853.
Vega SL, Liu E, Patel PJ, Kulesa AB, Carlson AL, Ma Y, et al. High-content imaging-based screening of microenvironment-induced changes to stem cells. Journal of biomolecular screening. 2012 Oct;17(9):1151–62.
Vega, Sebastián L., et al. “High-content imaging-based screening of microenvironment-induced changes to stem cells.Journal of Biomolecular Screening, vol. 17, no. 9, Oct. 2012, pp. 1151–62. Epmc, doi:10.1177/1087057112453853.
Vega SL, Liu E, Patel PJ, Kulesa AB, Carlson AL, Ma Y, Becker ML, Moghe PV. High-content imaging-based screening of microenvironment-induced changes to stem cells. Journal of biomolecular screening. 2012 Oct;17(9):1151–1162.
Journal cover image

Published In

Journal of biomolecular screening

DOI

EISSN

1552-454X

ISSN

1087-0571

Publication Date

October 2012

Volume

17

Issue

9

Start / End Page

1151 / 1162

Related Subject Headings

  • Tissue Scaffolds
  • SOXB1 Transcription Factors
  • Principal Component Analysis
  • Polyethylene Glycols
  • Osteogenesis
  • Nuclear Matrix-Associated Proteins
  • Microscopy, Confocal
  • Mesenchymal Stem Cells
  • Medicinal & Biomolecular Chemistry
  • Induced Pluripotent Stem Cells