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The use of immobilized osteogenic growth peptide on gradient substrates synthesized via click chemistry to enhance MC3T3-E1 osteoblast proliferation.

Publication ,  Journal Article
Moore, NM; Lin, NJ; Gallant, ND; Becker, ML
Published in: Biomaterials
March 2010

In this study, we report the use of surface immobilized peptide concentration gradient technology to characterize MC3T3-E1 osteoblast cell response to osteogenic growth peptide (OGP), a small peptide found naturally in human serum at mumol/L concentrations. OGP was coupled to oxidized self assembled monolayer (SAM) gradients by a polyethylene oxide (PEO) linker using click chemistry. After 4h incubation with MC3T3-E1 cells, OGP functionalized surfaces had higher cell attachment at low peptide concentrations compared to control gradients. By day 3, OGP gradient substrates had higher cell densities compared to control gradients at all concentrations. MC3T3-E1 cell doubling time was 35% faster on OGP substrates relative to SAM gradients alone, signifying an appreciable increase in cell proliferation. This increase in cell proliferation, or decrease in doubling time, due to OGP peptide was reduced by day 7. Hence, immobilized OGP increased cell proliferation from 0 days to 3 days at all densities indicating it may be useful as a proliferative peptide that can be used in tissue engineering substrates.

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

March 2010

Volume

31

Issue

7

Start / End Page

1604 / 1611

Related Subject Headings

  • Surface Properties
  • Substrate Specificity
  • Osteoblasts
  • Mice
  • Intercellular Signaling Peptides and Proteins
  • Immobilized Proteins
  • Humans
  • Histones
  • Gene Expression Regulation
  • Core Binding Factor Alpha 1 Subunit
 

Citation

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MLA
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Moore, N. M., Lin, N. J., Gallant, N. D., & Becker, M. L. (2010). The use of immobilized osteogenic growth peptide on gradient substrates synthesized via click chemistry to enhance MC3T3-E1 osteoblast proliferation. Biomaterials, 31(7), 1604–1611. https://doi.org/10.1016/j.biomaterials.2009.11.011
Moore, Nicole M., Nancy J. Lin, Nathan D. Gallant, and Matthew L. Becker. “The use of immobilized osteogenic growth peptide on gradient substrates synthesized via click chemistry to enhance MC3T3-E1 osteoblast proliferation.Biomaterials 31, no. 7 (March 2010): 1604–11. https://doi.org/10.1016/j.biomaterials.2009.11.011.
Moore, Nicole M., et al. “The use of immobilized osteogenic growth peptide on gradient substrates synthesized via click chemistry to enhance MC3T3-E1 osteoblast proliferation.Biomaterials, vol. 31, no. 7, Mar. 2010, pp. 1604–11. Epmc, doi:10.1016/j.biomaterials.2009.11.011.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

March 2010

Volume

31

Issue

7

Start / End Page

1604 / 1611

Related Subject Headings

  • Surface Properties
  • Substrate Specificity
  • Osteoblasts
  • Mice
  • Intercellular Signaling Peptides and Proteins
  • Immobilized Proteins
  • Humans
  • Histones
  • Gene Expression Regulation
  • Core Binding Factor Alpha 1 Subunit