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Macrophage response to methacrylate conversion using a gradient approach.

Publication ,  Journal Article
Lin, NJ; Bailey, LO; Becker, ML; Washburn, NR; Henderson, LA
Published in: Acta biomaterialia
March 2007

Incomplete conversion, an ongoing challenge facing photopolymerized methacrylate-based polymers, affects leachables as well as the resulting polymer network. As novel polymers and composites are developed, methods to efficiently screen cell response to these materials and their properties, including conversion, are needed. In this study, an in vitro screening methodology was developed to assess cells cultured directly on cross-linked polymer networks. A gradient in methacrylate double bond conversion was used to increase the experimental throughput. A substrate of 2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy)phenyl] propane (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) was prepared with a conversion ranging from 43.0% to 61.2%. Substrates aged for 7 days had no significant differences in surface roughness or hydrophilicity as a function of conversion. Leachables were detectable for at least 7 days using UV absorption, but their global cytotoxicity was insignificant after 5 days of aging. Thus, RAW 264.7 macrophage-like cells were cultured on aged substrates to evaluate the cell response to conversion, with possible contributions from the polymer network and local leachables. Conversions of 45% and 50% decreased viability (via calcein/ethidium staining) and increased apoptosis (via annexin-V staining). No significant changes (p>0.05) in tumor necrosis factor-alpha and interleukin-1beta gene expression, as measured by quantitative, real-time reverse transcription-polymerase chain reaction, were seen as conversion increased. Thus, conversions greater than 50% are recommended for equimolar BisGMA/TEGDMA. The ability to distinguish cell response as a function of conversion is useful as an initial biological screening platform to optimize dental polymers.

Duke Scholars

Published In

Acta biomaterialia

DOI

EISSN

1878-7568

ISSN

1742-7061

Publication Date

March 2007

Volume

3

Issue

2

Start / End Page

163 / 173

Related Subject Headings

  • Time Factors
  • Spectroscopy, Near-Infrared
  • Reverse Transcriptase Polymerase Chain Reaction
  • Polymethacrylic Acids
  • Polymers
  • Polyethylene Glycols
  • Mice
  • Methacrylates
  • Macrophages
  • Inflammation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lin, N. J., Bailey, L. O., Becker, M. L., Washburn, N. R., & Henderson, L. A. (2007). Macrophage response to methacrylate conversion using a gradient approach. Acta Biomaterialia, 3(2), 163–173. https://doi.org/10.1016/j.actbio.2006.10.001
Lin, Nancy J., LeeAnn O. Bailey, Matthew L. Becker, Newell R. Washburn, and Lori A. Henderson. “Macrophage response to methacrylate conversion using a gradient approach.Acta Biomaterialia 3, no. 2 (March 2007): 163–73. https://doi.org/10.1016/j.actbio.2006.10.001.
Lin NJ, Bailey LO, Becker ML, Washburn NR, Henderson LA. Macrophage response to methacrylate conversion using a gradient approach. Acta biomaterialia. 2007 Mar;3(2):163–73.
Lin, Nancy J., et al. “Macrophage response to methacrylate conversion using a gradient approach.Acta Biomaterialia, vol. 3, no. 2, Mar. 2007, pp. 163–73. Epmc, doi:10.1016/j.actbio.2006.10.001.
Lin NJ, Bailey LO, Becker ML, Washburn NR, Henderson LA. Macrophage response to methacrylate conversion using a gradient approach. Acta biomaterialia. 2007 Mar;3(2):163–173.
Journal cover image

Published In

Acta biomaterialia

DOI

EISSN

1878-7568

ISSN

1742-7061

Publication Date

March 2007

Volume

3

Issue

2

Start / End Page

163 / 173

Related Subject Headings

  • Time Factors
  • Spectroscopy, Near-Infrared
  • Reverse Transcriptase Polymerase Chain Reaction
  • Polymethacrylic Acids
  • Polymers
  • Polyethylene Glycols
  • Mice
  • Methacrylates
  • Macrophages
  • Inflammation