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Cellular response to phase-separated blends of tyrosine-derived polycarbonates.

Publication ,  Journal Article
Bailey, LO; Becker, ML; Stephens, JS; Gallant, ND; Mahoney, CM; Washburn, NR; Rege, A; Kohn, J; Amis, EJ
Published in: Journal of biomedical materials research. Part A
March 2006

Two-dimensional thin films consisting of homopolymer and discrete compositional blends of tyrosine-derived polycarbonates were prepared and characterized in an effort to elucidate the nature of different cell responses that were measured in vitro. The structurally similar blends were found to phase separate after annealing with domain sizes dependent on the overall composition. The thin polymer films were characterized with the use of atomic force microscopy (AFM), water contact angles, and time-of-flight secondary ion mass spectrometry (TOF-SIMS) and significant changes in roughness were measured following the annealing process. Genetic expression profiles of interleukin-1beta and fibronectin in MC3T3-E1 osteoblasts and RAW 264.7 murine macrophages were measured at several time points, demonstrating the time and composition-dependent nature of the cell responses. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) depicted upregulation of the fibronectin gene copy numbers in each of the blends relative to the homopolymers. Moreover, the interleukin-1beta expression profile was found to be compositionally dependent. The data suggest strongly that optimal composition and processing conditions can significantly affect the acute inflammatory and extracellular matrix production responses.

Duke Scholars

Published In

Journal of biomedical materials research. Part A

DOI

EISSN

1552-4965

ISSN

1549-3296

Publication Date

March 2006

Volume

76

Issue

3

Start / End Page

491 / 502

Related Subject Headings

  • Tyrosine
  • Polycarboxylate Cement
  • Osteoblasts
  • Mice
  • Membranes, Artificial
  • Materials Testing
  • Mass Spectrometry
  • Macrophages
  • Interleukin-1
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bailey, L. O., Becker, M. L., Stephens, J. S., Gallant, N. D., Mahoney, C. M., Washburn, N. R., … Amis, E. J. (2006). Cellular response to phase-separated blends of tyrosine-derived polycarbonates. Journal of Biomedical Materials Research. Part A, 76(3), 491–502. https://doi.org/10.1002/jbm.a.30527
Bailey, LeeAnn O., Matthew L. Becker, Jean S. Stephens, Nathan D. Gallant, Christine M. Mahoney, Newell R. Washburn, Aarti Rege, Joachim Kohn, and Eric J. Amis. “Cellular response to phase-separated blends of tyrosine-derived polycarbonates.Journal of Biomedical Materials Research. Part A 76, no. 3 (March 2006): 491–502. https://doi.org/10.1002/jbm.a.30527.
Bailey LO, Becker ML, Stephens JS, Gallant ND, Mahoney CM, Washburn NR, et al. Cellular response to phase-separated blends of tyrosine-derived polycarbonates. Journal of biomedical materials research Part A. 2006 Mar;76(3):491–502.
Bailey, LeeAnn O., et al. “Cellular response to phase-separated blends of tyrosine-derived polycarbonates.Journal of Biomedical Materials Research. Part A, vol. 76, no. 3, Mar. 2006, pp. 491–502. Epmc, doi:10.1002/jbm.a.30527.
Bailey LO, Becker ML, Stephens JS, Gallant ND, Mahoney CM, Washburn NR, Rege A, Kohn J, Amis EJ. Cellular response to phase-separated blends of tyrosine-derived polycarbonates. Journal of biomedical materials research Part A. 2006 Mar;76(3):491–502.
Journal cover image

Published In

Journal of biomedical materials research. Part A

DOI

EISSN

1552-4965

ISSN

1549-3296

Publication Date

March 2006

Volume

76

Issue

3

Start / End Page

491 / 502

Related Subject Headings

  • Tyrosine
  • Polycarboxylate Cement
  • Osteoblasts
  • Mice
  • Membranes, Artificial
  • Materials Testing
  • Mass Spectrometry
  • Macrophages
  • Interleukin-1
  • Humans