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Mechanically tunable thin films of photosensitive artificial proteins: Preparation and characterization by nanoindentation

Publication ,  Journal Article
Nowatzki, PJ; Franck, C; Maskarinec, SA; Ravichandran, G; Tirrell, DA
Published in: Macromolecules
March 11, 2008
Published version (DOI)

Thin films of controlled elastic modulus were made by photo-cross-linking artificial extracellular matrix (aECM) proteins containing the photosensitive amino acid p-azidophenylalanine (pN3Phe). The elastic moduli of the films were calculated from nanoindentation data collected by atomic force microscopy (AFM) using a thin-film Hertz model. The modulus was shown to be tunable in the range 0.3-1.0 MPa either by controlling the irradiation time or by varying the level of pN3Phe in the protein. Tensile measurements on bulk films of the same proteins and finite-element simulation of the indentation process agreed with the thin-film modulus measurements from AFM. Substrates characterized by spatial variation in elastic modulus were created by local control of the irradiation time. © 2008 American Chemical Society.

Duke Scholars

Stacey Ann Maskarinec
Author Stacey Ann Maskarinec Medicine, Infectious Diseases

Published In

Macromolecules

DOI

10.1021/ma071717a

ISSN

0024-9297

Publication Date

March 11, 2008

Volume

41

Issue

5

Start / End Page

1839 / 1845

Related Subject Headings

  • Polymers
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Nowatzki, P. J., Franck, C., Maskarinec, S. A., Ravichandran, G., & Tirrell, D. A. (2008). Mechanically tunable thin films of photosensitive artificial proteins: Preparation and characterization by nanoindentation. Macromolecules, 41(5), 1839–1845. https://doi.org/10.1021/ma071717a
Nowatzki, P. J., C. Franck, S. A. Maskarinec, G. Ravichandran, and D. A. Tirrell. “Mechanically tunable thin films of photosensitive artificial proteins: Preparation and characterization by nanoindentation.” Macromolecules 41, no. 5 (March 11, 2008): 1839–45. https://doi.org/10.1021/ma071717a.
Nowatzki PJ, Franck C, Maskarinec SA, Ravichandran G, Tirrell DA. Mechanically tunable thin films of photosensitive artificial proteins: Preparation and characterization by nanoindentation. Macromolecules. 2008 Mar 11;41(5):1839–45.
Nowatzki, P. J., et al. “Mechanically tunable thin films of photosensitive artificial proteins: Preparation and characterization by nanoindentation.” Macromolecules, vol. 41, no. 5, Mar. 2008, pp. 1839–45. Scopus, doi:10.1021/ma071717a.
Nowatzki PJ, Franck C, Maskarinec SA, Ravichandran G, Tirrell DA. Mechanically tunable thin films of photosensitive artificial proteins: Preparation and characterization by nanoindentation. Macromolecules. 2008 Mar 11;41(5):1839–1845.
Journal cover image

Published In

Macromolecules

DOI

10.1021/ma071717a

ISSN

0024-9297

Publication Date

March 11, 2008

Volume

41

Issue

5

Start / End Page

1839 / 1845

Related Subject Headings

  • Polymers
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences