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Human gut microbiota is associated with HIV-reactive immunoglobulin at baseline and following HIV vaccination.

Publication ,  Journal Article
Cram, JA; Fiore-Gartland, AJ; Srinivasan, S; Karuna, S; Pantaleo, G; Tomaras, GD; Fredricks, DN; Kublin, JG
Published in: PLoS One
2019

Antibodies that recognize commensal microbial antigens may be cross reactive with a part of the human immunodeficiency virus (HIV) envelope glycoprotein gp41. To improve understanding of the role of the microbiota in modulating the immune response to HIV vaccines, we studied the associations of the gut microbiota composition of participants in the HIV Vaccine Trials Network 096 clinical trial with their HIV-specific immune responses in response to vaccination with a DNA-prime, pox virus boost strategy designed to recapitulate the only efficacious HIV-vaccine trial (RV144). We observed that both levels of IgG antibodies to gp41 at baseline and post-vaccination levels of IgG antibodies to the Con.6.gp120.B, ZM96.gp140 and gp70 B.CaseA V1-V2 antigens were associated with three co-occurring clusters of family level microbial taxa. One cluster contained several families positively associated with gp41-specific IgG and negatively associated with vaccine-matched gp120, gp140 and V1-V2-specific IgG responses. A second cluster contained families that negatively associated with gp41 and positively associated with gp120, gp140 and V1-V2-specific IgG responses. A third cluster contained microbial groups that did not correlate with any immune responses. Baseline and post-vaccination levels of gp41 IgG were not significantly correlated, suggesting that factors beyond the microbiome that contribute to immune response heterogeneity. Sequence variant richness was positively associated with gp41, p24, pg140 and V1-V2 specific IgG responses, gp41 and p24 IgA responses, and CD4+ T cell responses to HIV-1 proteins. Our findings provide preliminary evidence that the gut microbiota may be an important predictor of vaccine response.

Duke Scholars

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2019

Volume

14

Issue

12

Start / End Page

e0225622

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Young Adult
  • Viral Vaccines
  • Vaccinia virus
  • Vaccines, Attenuated
  • Vaccination
  • Middle Aged
  • Male
  • Immunogenicity, Vaccine
  • Humans
 

Citation

APA
Chicago
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MLA
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Cram, J. A., Fiore-Gartland, A. J., Srinivasan, S., Karuna, S., Pantaleo, G., Tomaras, G. D., … Kublin, J. G. (2019). Human gut microbiota is associated with HIV-reactive immunoglobulin at baseline and following HIV vaccination. PLoS One, 14(12), e0225622. https://doi.org/10.1371/journal.pone.0225622
Cram, Jacob A., Andrew J. Fiore-Gartland, Sujatha Srinivasan, Shelly Karuna, Giuseppe Pantaleo, Georgia D. Tomaras, David N. Fredricks, and James G. Kublin. “Human gut microbiota is associated with HIV-reactive immunoglobulin at baseline and following HIV vaccination.PLoS One 14, no. 12 (2019): e0225622. https://doi.org/10.1371/journal.pone.0225622.
Cram JA, Fiore-Gartland AJ, Srinivasan S, Karuna S, Pantaleo G, Tomaras GD, et al. Human gut microbiota is associated with HIV-reactive immunoglobulin at baseline and following HIV vaccination. PLoS One. 2019;14(12):e0225622.
Cram, Jacob A., et al. “Human gut microbiota is associated with HIV-reactive immunoglobulin at baseline and following HIV vaccination.PLoS One, vol. 14, no. 12, 2019, p. e0225622. Pubmed, doi:10.1371/journal.pone.0225622.
Cram JA, Fiore-Gartland AJ, Srinivasan S, Karuna S, Pantaleo G, Tomaras GD, Fredricks DN, Kublin JG. Human gut microbiota is associated with HIV-reactive immunoglobulin at baseline and following HIV vaccination. PLoS One. 2019;14(12):e0225622.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2019

Volume

14

Issue

12

Start / End Page

e0225622

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Young Adult
  • Viral Vaccines
  • Vaccinia virus
  • Vaccines, Attenuated
  • Vaccination
  • Middle Aged
  • Male
  • Immunogenicity, Vaccine
  • Humans