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Molecular and clonal evolution in recurrent metastatic gliosarcoma.

Publication ,  Journal Article
Anderson, KJ; Tan, AC; Parkinson, J; Back, M; Kastelan, M; Newey, A; Brewer, J; Wheeler, H; Hudson, AL; Amin, SB; Johnson, KC; Barthel, FP ...
Published in: Cold Spring Harb Mol Case Stud
February 2020

We discuss the molecular evolution of gliosarcoma, a mesenchymal type of glioblastoma (GBM), using the case of a 37-yr-old woman who developed two recurrences and an extracranial metastasis. She was initially diagnosed with isocitrate dehydrogenase (IDH) wild-type gliosarcoma in the frontal lobe and treated with surgery followed by concurrent radiotherapy with temozolomide. Five months later the tumor recurred in the left frontal lobe, outside the initially resected area, and was treated with further surgery and radiotherapy. Six months later the patient developed a second left frontal recurrence and was again treated with surgery and radiotherapy. Six weeks later, further recurrence was observed in the brain and bone, and biopsy confirmed metastases in the pelvic bones. To understand the clonal relationships between the four tumor instances and the origin of metastasis, we performed whole-genome sequencing of the intracranial tumors and the tumor located in the right iliac bone. We compared their mutational and copy-number profiles and inferred the clonal phylogeny. The tumors harbored shared alterations in GBM driver genes, including mutations in TP53, NF1, and RB1, and CDKN2A deletion. Whole-genome doubling was identified in the first recurrence and the extracranial metastasis. Comparisons of the metastatic to intracranial tumors highlighted a high similarity in molecular profile but contrasting evidence regarding the origin of the metastasis. Subclonal reconstruction suggested a parallel evolution of the recurrent tumors, and that the metastatic tumor was largely derived from the first recurrence. We conclude that metastasis in glioma can be a late event in tumorigenesis.

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Published In

Cold Spring Harb Mol Case Stud

DOI

EISSN

2373-2873

Publication Date

February 2020

Volume

6

Issue

1

Location

United States

Related Subject Headings

  • Recurrence
  • Neoplasm Staging
  • Neoplasm Metastasis
  • Mutation
  • Multimodal Imaging
  • Immunohistochemistry
  • Humans
  • Gliosarcoma
  • Female
  • DNA Copy Number Variations
 

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Anderson, K. J., Tan, A. C., Parkinson, J., Back, M., Kastelan, M., Newey, A., … Khasraw, M. (2020). Molecular and clonal evolution in recurrent metastatic gliosarcoma. Cold Spring Harb Mol Case Stud, 6(1). https://doi.org/10.1101/mcs.a004671
Anderson, Kevin J., Aaron C. Tan, Jonathon Parkinson, Michael Back, Marina Kastelan, Allison Newey, Janice Brewer, et al. “Molecular and clonal evolution in recurrent metastatic gliosarcoma.Cold Spring Harb Mol Case Stud 6, no. 1 (February 2020). https://doi.org/10.1101/mcs.a004671.
Anderson KJ, Tan AC, Parkinson J, Back M, Kastelan M, Newey A, et al. Molecular and clonal evolution in recurrent metastatic gliosarcoma. Cold Spring Harb Mol Case Stud. 2020 Feb;6(1).
Anderson, Kevin J., et al. “Molecular and clonal evolution in recurrent metastatic gliosarcoma.Cold Spring Harb Mol Case Stud, vol. 6, no. 1, Feb. 2020. Pubmed, doi:10.1101/mcs.a004671.
Anderson KJ, Tan AC, Parkinson J, Back M, Kastelan M, Newey A, Brewer J, Wheeler H, Hudson AL, Amin SB, Johnson KC, Barthel FP, Verhaak RGW, Khasraw M. Molecular and clonal evolution in recurrent metastatic gliosarcoma. Cold Spring Harb Mol Case Stud. 2020 Feb;6(1).

Published In

Cold Spring Harb Mol Case Stud

DOI

EISSN

2373-2873

Publication Date

February 2020

Volume

6

Issue

1

Location

United States

Related Subject Headings

  • Recurrence
  • Neoplasm Staging
  • Neoplasm Metastasis
  • Mutation
  • Multimodal Imaging
  • Immunohistochemistry
  • Humans
  • Gliosarcoma
  • Female
  • DNA Copy Number Variations