
Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection
Publication
, Journal Article
Zimmermann, RM; Schmidt, CF; Gaub, HE
Published in: Journal of Colloid And Interface Science
October 1, 1990
A combination of total internal reflection fluorescence and fluorescence recovery after photobleaching was used to measure the adsorption of 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD) labeled bovine serum albumin and NBD labeled lysozyme to plain quartz and alkylated quartz. A new method was developed to measure quantitatively the adsorbed amount of protein, using an intrinsic calibration. The results were confirmed by accompanying ellipsometry measurements. Equilibrium exchange kinetics of the adsorbed proteins between the surface and the bulk were measured as a function of temperature. © 1990.
Duke Scholars
Published In
Journal of Colloid And Interface Science
DOI
ISSN
0021-9797
Publication Date
October 1, 1990
Volume
139
Issue
1
Start / End Page
268 / 280
Related Subject Headings
- Chemical Physics
- 51 Physical sciences
- 40 Engineering
- 34 Chemical sciences
- 09 Engineering
- 03 Chemical Sciences
- 02 Physical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Zimmermann, R. M., Schmidt, C. F., & Gaub, H. E. (1990). Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection. Journal of Colloid And Interface Science, 139(1), 268–280. https://doi.org/10.1016/0021-9797(90)90462-W
Zimmermann, R. M., C. F. Schmidt, and H. E. Gaub. “Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection.” Journal of Colloid And Interface Science 139, no. 1 (October 1, 1990): 268–80. https://doi.org/10.1016/0021-9797(90)90462-W.
Zimmermann RM, Schmidt CF, Gaub HE. Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection. Journal of Colloid And Interface Science. 1990 Oct 1;139(1):268–80.
Zimmermann, R. M., et al. “Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection.” Journal of Colloid And Interface Science, vol. 139, no. 1, Oct. 1990, pp. 268–80. Scopus, doi:10.1016/0021-9797(90)90462-W.
Zimmermann RM, Schmidt CF, Gaub HE. Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection. Journal of Colloid And Interface Science. 1990 Oct 1;139(1):268–280.

Published In
Journal of Colloid And Interface Science
DOI
ISSN
0021-9797
Publication Date
October 1, 1990
Volume
139
Issue
1
Start / End Page
268 / 280
Related Subject Headings
- Chemical Physics
- 51 Physical sciences
- 40 Engineering
- 34 Chemical sciences
- 09 Engineering
- 03 Chemical Sciences
- 02 Physical Sciences