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Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection

Publication ,  Journal Article
Zimmermann, RM; Schmidt, CF; Gaub, HE
Published in: Journal of Colloid And Interface Science
October 1, 1990

A combination of total internal reflection fluorescence and fluorescence recovery after photobleaching was used to measure the adsorption of 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD) labeled bovine serum albumin and NBD labeled lysozyme to plain quartz and alkylated quartz. A new method was developed to measure quantitatively the adsorbed amount of protein, using an intrinsic calibration. The results were confirmed by accompanying ellipsometry measurements. Equilibrium exchange kinetics of the adsorbed proteins between the surface and the bulk were measured as a function of temperature. © 1990.

Duke Scholars

Published In

Journal of Colloid And Interface Science

DOI

ISSN

0021-9797

Publication Date

October 1, 1990

Volume

139

Issue

1

Start / End Page

268 / 280

Related Subject Headings

  • Chemical Physics
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences
  • 02 Physical Sciences
 

Citation

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Zimmermann, R. M., Schmidt, C. F., & Gaub, H. E. (1990). Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection. Journal of Colloid And Interface Science, 139(1), 268–280. https://doi.org/10.1016/0021-9797(90)90462-W
Zimmermann, R. M., C. F. Schmidt, and H. E. Gaub. “Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection.” Journal of Colloid And Interface Science 139, no. 1 (October 1, 1990): 268–80. https://doi.org/10.1016/0021-9797(90)90462-W.
Zimmermann RM, Schmidt CF, Gaub HE. Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection. Journal of Colloid And Interface Science. 1990 Oct 1;139(1):268–80.
Zimmermann, R. M., et al. “Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection.” Journal of Colloid And Interface Science, vol. 139, no. 1, Oct. 1990, pp. 268–80. Scopus, doi:10.1016/0021-9797(90)90462-W.
Zimmermann RM, Schmidt CF, Gaub HE. Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection. Journal of Colloid And Interface Science. 1990 Oct 1;139(1):268–280.
Journal cover image

Published In

Journal of Colloid And Interface Science

DOI

ISSN

0021-9797

Publication Date

October 1, 1990

Volume

139

Issue

1

Start / End Page

268 / 280

Related Subject Headings

  • Chemical Physics
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences
  • 02 Physical Sciences