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Optical trap stiffness in the presence and absence of spherical aberrations.

Publication ,  Journal Article
Vermeulen, KC; Wuite, GJL; Stienen, GJM; Schmidt, CF
Published in: Applied optics
March 2006
Published version (DOI)

Optical traps are commonly constructed with high-numerical-aperture objectives. Oil-immersion objectives suffer from spherical aberrations when used for imaging in aqueous solutions. The effect of spherical aberrations on trapping strength has been modeled by approximation, and only a few experimental results are available in the case of micrometer-sized particles. We present an experimental study of the dependence of lateral and axial optical-trap stiffness on focusing depth for polystyrene and silica beads of 2 microm diameter by using oil- and water-immersion objectives. We demonstrate a strong depth dependence of trap stiffness with the oil-immersion objective, whereas no depth dependence was observed with the water-immersion objective.

Duke Scholars

Christoph F. Schmidt
Author Christoph F. Schmidt Physics

Published In

Applied optics

DOI

10.1364/ao.45.001812

EISSN

1539-4522

ISSN

1559-128X

Publication Date

March 2006

Volume

45

Issue

8

Start / End Page

1812 / 1819

Related Subject Headings

  • Optics
  • 5102 Atomic, molecular and optical physics
  • 4008 Electrical engineering
  • 0913 Mechanical Engineering
  • 0906 Electrical and Electronic Engineering
  • 0205 Optical Physics
 

Citation

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ICMJE
MLA
NLM
Vermeulen, K. C., Wuite, G. J. L., Stienen, G. J. M., & Schmidt, C. F. (2006). Optical trap stiffness in the presence and absence of spherical aberrations. Applied Optics, 45(8), 1812–1819. https://doi.org/10.1364/ao.45.001812
Vermeulen, Karen C., Gijs J. L. Wuite, Ger J. M. Stienen, and Christoph F. Schmidt. “Optical trap stiffness in the presence and absence of spherical aberrations.” Applied Optics 45, no. 8 (March 2006): 1812–19. https://doi.org/10.1364/ao.45.001812.
Vermeulen KC, Wuite GJL, Stienen GJM, Schmidt CF. Optical trap stiffness in the presence and absence of spherical aberrations. Applied optics. 2006 Mar;45(8):1812–9.
Vermeulen, Karen C., et al. “Optical trap stiffness in the presence and absence of spherical aberrations.” Applied Optics, vol. 45, no. 8, Mar. 2006, pp. 1812–19. Epmc, doi:10.1364/ao.45.001812.
Vermeulen KC, Wuite GJL, Stienen GJM, Schmidt CF. Optical trap stiffness in the presence and absence of spherical aberrations. Applied optics. 2006 Mar;45(8):1812–1819.

Published In

Applied optics

DOI

10.1364/ao.45.001812

EISSN

1539-4522

ISSN

1559-128X

Publication Date

March 2006

Volume

45

Issue

8

Start / End Page

1812 / 1819

Related Subject Headings

  • Optics
  • 5102 Atomic, molecular and optical physics
  • 4008 Electrical engineering
  • 0913 Mechanical Engineering
  • 0906 Electrical and Electronic Engineering
  • 0205 Optical Physics