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Force fluctuations in three-dimensional suspended fibroblasts.

Publication ,  Journal Article
Schlosser, F; Rehfeldt, F; Schmidt, CF
Published in: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
February 2015

Cells are sensitive to mechanical cues from their environment and at the same time generate and transmit forces to their surroundings. To test quantitatively forces generated by cells not attached to a substrate, we used a dual optical trap to suspend 3T3 fibroblasts between two fibronectin-coated beads. In this simple geometry, we measured both the cells' elastic properties and the force fluctuations they generate with high bandwidth. Cell stiffness decreased substantially with both myosin inhibition by blebbistatin and serum-starvation, but not with microtubule depolymerization by nocodazole. We show that cortical forces generated by non-muscle myosin II deform the cell from its rounded shape in the frequency regime from 0.1 to 10 Hz. The amplitudes of these forces were strongly reduced by blebbistatin and serum starvation, but were unaffected by depolymerization of microtubules. Force fluctuations show a spectrum that is characteristic for an elastic network activated by random sustained stresses with abrupt transitions.

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Published In

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences

DOI

EISSN

1471-2970

ISSN

0962-8436

Publication Date

February 2015

Volume

370

Issue

1661

Start / End Page

20140028

Related Subject Headings

  • Surface Tension
  • Myosins
  • Mice
  • Fibroblasts
  • Evolutionary Biology
  • Cell Adhesion
  • Biomechanical Phenomena
  • Animals
  • Actins
  • 3T3 Cells
 

Citation

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Schlosser, F., Rehfeldt, F., & Schmidt, C. F. (2015). Force fluctuations in three-dimensional suspended fibroblasts. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, 370(1661), 20140028. https://doi.org/10.1098/rstb.2014.0028
Schlosser, Florian, Florian Rehfeldt, and Christoph F. Schmidt. “Force fluctuations in three-dimensional suspended fibroblasts.Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 370, no. 1661 (February 2015): 20140028. https://doi.org/10.1098/rstb.2014.0028.
Schlosser F, Rehfeldt F, Schmidt CF. Force fluctuations in three-dimensional suspended fibroblasts. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences. 2015 Feb;370(1661):20140028.
Schlosser, Florian, et al. “Force fluctuations in three-dimensional suspended fibroblasts.Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, vol. 370, no. 1661, Feb. 2015, p. 20140028. Epmc, doi:10.1098/rstb.2014.0028.
Schlosser F, Rehfeldt F, Schmidt CF. Force fluctuations in three-dimensional suspended fibroblasts. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences. 2015 Feb;370(1661):20140028.
Journal cover image

Published In

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences

DOI

EISSN

1471-2970

ISSN

0962-8436

Publication Date

February 2015

Volume

370

Issue

1661

Start / End Page

20140028

Related Subject Headings

  • Surface Tension
  • Myosins
  • Mice
  • Fibroblasts
  • Evolutionary Biology
  • Cell Adhesion
  • Biomechanical Phenomena
  • Animals
  • Actins
  • 3T3 Cells