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Imaging microtubules and kinesin decorated microtubules using tapping mode atomic force microscopy in fluids.

Publication ,  Journal Article
Kacher, CM; Weiss, IM; Stewart, RJ; Schmidt, CF; Hansma, PK; Radmacher, M; Fritz, M
Published in: European biophysics journal : EBJ
January 2000

The atomic force microscope has been used to investigate microtubules and kinesin decorated microtubules in aqueous solution adsorbed onto a solid substrate. The netto negatively charged microtubules did not adsorb to negatively charged solid surfaces but to glass covalently coated with the highly positively charged silane trimethoxysilylpropyldiethylenetriamine (DETA) or a lipid bilayer of 1,2-dipalmitoyl-3-dimethylammoniumpropane. Using electron beam deposited tips for microtubules adsorbed on DETA, single protofilaments could be observed showing that the resolution is up to 5 nm. Under conditions where the silane coated surfaces are hydrophobic, microtubules opened, presumably at the seam, whose stability is lower than that of the bonds between the other protofilaments. This led to a "sheet" with a width of about 100 nm firmly attached to the surface. Microtubules decorated with a stoichiometric low amount of kinesin molecules in the presence of the non-hydrolyzable ATP-analog 5'-adenylylimidodiphosphate could also be adsorbed onto silane-coated glass. Imaging was very stable and the molecules did not show any scan-induced deformation even after hundreds of scans with a scan frequency of 100 Hz.

Duke Scholars

Published In

European biophysics journal : EBJ

DOI

EISSN

1432-1017

ISSN

0175-7571

Publication Date

January 2000

Volume

28

Issue

8

Start / End Page

611 / 620

Related Subject Headings

  • Water
  • Swine
  • Solutions
  • Microtubules
  • Microscopy, Atomic Force
  • Lipid Bilayers
  • Kinesins
  • Indicators and Reagents
  • Brain
  • Biophysics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kacher, C. M., Weiss, I. M., Stewart, R. J., Schmidt, C. F., Hansma, P. K., Radmacher, M., & Fritz, M. (2000). Imaging microtubules and kinesin decorated microtubules using tapping mode atomic force microscopy in fluids. European Biophysics Journal : EBJ, 28(8), 611–620. https://doi.org/10.1007/s002490050001
Kacher, C. M., I. M. Weiss, R. J. Stewart, C. F. Schmidt, P. K. Hansma, M. Radmacher, and M. Fritz. “Imaging microtubules and kinesin decorated microtubules using tapping mode atomic force microscopy in fluids.European Biophysics Journal : EBJ 28, no. 8 (January 2000): 611–20. https://doi.org/10.1007/s002490050001.
Kacher CM, Weiss IM, Stewart RJ, Schmidt CF, Hansma PK, Radmacher M, et al. Imaging microtubules and kinesin decorated microtubules using tapping mode atomic force microscopy in fluids. European biophysics journal : EBJ. 2000 Jan;28(8):611–20.
Kacher, C. M., et al. “Imaging microtubules and kinesin decorated microtubules using tapping mode atomic force microscopy in fluids.European Biophysics Journal : EBJ, vol. 28, no. 8, Jan. 2000, pp. 611–20. Epmc, doi:10.1007/s002490050001.
Kacher CM, Weiss IM, Stewart RJ, Schmidt CF, Hansma PK, Radmacher M, Fritz M. Imaging microtubules and kinesin decorated microtubules using tapping mode atomic force microscopy in fluids. European biophysics journal : EBJ. 2000 Jan;28(8):611–620.
Journal cover image

Published In

European biophysics journal : EBJ

DOI

EISSN

1432-1017

ISSN

0175-7571

Publication Date

January 2000

Volume

28

Issue

8

Start / End Page

611 / 620

Related Subject Headings

  • Water
  • Swine
  • Solutions
  • Microtubules
  • Microscopy, Atomic Force
  • Lipid Bilayers
  • Kinesins
  • Indicators and Reagents
  • Brain
  • Biophysics