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Drebrin-like protein DBN-1 is a sarcomere component that stabilizes actin filaments during muscle contraction.

Publication ,  Journal Article
Butkevich, E; Bodensiek, K; Fakhri, N; von Roden, K; Schaap, IAT; Majoul, I; Schmidt, CF; Klopfenstein, DR
Published in: Nature communications
July 2015

Actin filament organization and stability in the sarcomeres of muscle cells are critical for force generation. Here we identify and functionally characterize a Caenorhabditis elegans drebrin-like protein DBN-1 as a novel constituent of the muscle contraction machinery. In vitro, DBN-1 exhibits actin filament binding and bundling activity. In vivo, DBN-1 is expressed in body wall muscles of C. elegans. During the muscle contraction cycle, DBN-1 alternates location between myosin- and actin-rich regions of the sarcomere. In contracted muscle, DBN-1 is accumulated at I-bands where it likely regulates proper spacing of α-actinin and tropomyosin and protects actin filaments from the interaction with ADF/cofilin. DBN-1 loss of function results in the partial depolymerization of F-actin during muscle contraction. Taken together, our data show that DBN-1 organizes the muscle contractile apparatus maintaining the spatial relationship between actin-binding proteins such as α-actinin, tropomyosin and ADF/cofilin and possibly strengthening actin filaments by bundling.

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Published In

Nature communications

DOI

EISSN

2041-1723

ISSN

2041-1723

Publication Date

July 2015

Volume

6

Start / End Page

7523

Related Subject Headings

  • Sarcomeres
  • Promoter Regions, Genetic
  • Neuropeptides
  • Muscle, Skeletal
  • Muscle Contraction
  • Microscopy, Fluorescence
  • Gene Expression Regulation
  • Chlorocebus aethiops
  • Caenorhabditis elegans Proteins
  • Caenorhabditis elegans
 

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Butkevich, E., Bodensiek, K., Fakhri, N., von Roden, K., Schaap, I. A. T., Majoul, I., … Klopfenstein, D. R. (2015). Drebrin-like protein DBN-1 is a sarcomere component that stabilizes actin filaments during muscle contraction. Nature Communications, 6, 7523. https://doi.org/10.1038/ncomms8523
Butkevich, Eugenia, Kai Bodensiek, Nikta Fakhri, Kerstin von Roden, Iwan A. T. Schaap, Irina Majoul, Christoph F. Schmidt, and Dieter R. Klopfenstein. “Drebrin-like protein DBN-1 is a sarcomere component that stabilizes actin filaments during muscle contraction.Nature Communications 6 (July 2015): 7523. https://doi.org/10.1038/ncomms8523.
Butkevich E, Bodensiek K, Fakhri N, von Roden K, Schaap IAT, Majoul I, et al. Drebrin-like protein DBN-1 is a sarcomere component that stabilizes actin filaments during muscle contraction. Nature communications. 2015 Jul;6:7523.
Butkevich, Eugenia, et al. “Drebrin-like protein DBN-1 is a sarcomere component that stabilizes actin filaments during muscle contraction.Nature Communications, vol. 6, July 2015, p. 7523. Epmc, doi:10.1038/ncomms8523.
Butkevich E, Bodensiek K, Fakhri N, von Roden K, Schaap IAT, Majoul I, Schmidt CF, Klopfenstein DR. Drebrin-like protein DBN-1 is a sarcomere component that stabilizes actin filaments during muscle contraction. Nature communications. 2015 Jul;6:7523.

Published In

Nature communications

DOI

EISSN

2041-1723

ISSN

2041-1723

Publication Date

July 2015

Volume

6

Start / End Page

7523

Related Subject Headings

  • Sarcomeres
  • Promoter Regions, Genetic
  • Neuropeptides
  • Muscle, Skeletal
  • Muscle Contraction
  • Microscopy, Fluorescence
  • Gene Expression Regulation
  • Chlorocebus aethiops
  • Caenorhabditis elegans Proteins
  • Caenorhabditis elegans