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Protein tracking and detection of protein motion using atomic force microscopy.

Publication ,  Journal Article
Thomson, NH; Fritz, M; Radmacher, M; Cleveland, JP; Schmidt, CF; Hansma, PK
Published in: Biophysical journal
May 1996

Height fluctuations over three different proteins, immunoglobulin G, urease, and microtubules, have been measured using an atomic force microscope (AFM) operating in fluid tapping mode. This was achieved by using a protein-tracking system, where the AFM tip was periodically repositioned above a single protein molecule (or structure) as thermal drifting occurred. Height (z-piezo signal) data were taken in 1 - or 2-s time slices with the tip over the molecule and compared to data taken on the support. The measured fluctuations were consistently higher when the tip was positioned over the protein, as opposed to the support the protein was adsorbed on. Similar measurements over patches of an amphiphile, where the noise was identical to that on the support, suggest that the noise increase is due to some intrinsic property of proteins and is not a result of different tip-sample interactions over soft samples. The orientation of the adsorbed proteins in these preliminary studies was not known; thus it was not possible to make correlations between the observed motion and specific protein structure or protein function beyond noting that the observed height fluctuations were greater for an antibody (anti-bovine IgG) and an enzyme (urease) than for microtubules.

Duke Scholars

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

May 1996

Volume

70

Issue

5

Start / End Page

2421 / 2431

Related Subject Headings

  • Urease
  • Time Factors
  • Surface-Active Agents
  • Quaternary Ammonium Compounds
  • Microtubules
  • Microscopy, Atomic Force
  • Immunoglobulin G
  • Freeze Drying
  • Fourier Analysis
  • Cattle
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Thomson, N. H., Fritz, M., Radmacher, M., Cleveland, J. P., Schmidt, C. F., & Hansma, P. K. (1996). Protein tracking and detection of protein motion using atomic force microscopy. Biophysical Journal, 70(5), 2421–2431. https://doi.org/10.1016/s0006-3495(96)79812-0
Thomson, N. H., M. Fritz, M. Radmacher, J. P. Cleveland, C. F. Schmidt, and P. K. Hansma. “Protein tracking and detection of protein motion using atomic force microscopy.Biophysical Journal 70, no. 5 (May 1996): 2421–31. https://doi.org/10.1016/s0006-3495(96)79812-0.
Thomson NH, Fritz M, Radmacher M, Cleveland JP, Schmidt CF, Hansma PK. Protein tracking and detection of protein motion using atomic force microscopy. Biophysical journal. 1996 May;70(5):2421–31.
Thomson, N. H., et al. “Protein tracking and detection of protein motion using atomic force microscopy.Biophysical Journal, vol. 70, no. 5, May 1996, pp. 2421–31. Epmc, doi:10.1016/s0006-3495(96)79812-0.
Thomson NH, Fritz M, Radmacher M, Cleveland JP, Schmidt CF, Hansma PK. Protein tracking and detection of protein motion using atomic force microscopy. Biophysical journal. 1996 May;70(5):2421–2431.
Journal cover image

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

May 1996

Volume

70

Issue

5

Start / End Page

2421 / 2431

Related Subject Headings

  • Urease
  • Time Factors
  • Surface-Active Agents
  • Quaternary Ammonium Compounds
  • Microtubules
  • Microscopy, Atomic Force
  • Immunoglobulin G
  • Freeze Drying
  • Fourier Analysis
  • Cattle