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Microfluidic device for chemical and mechanical manipulation of suspended cells

Publication ,  Journal Article
Rezvani, S; Shi, N; Squires, TM; Schmidt, CF
Published in: Journal of Physics D: Applied Physics
January 10, 2018

Microfluidic devices have proven to be useful and versatile for cell studies. We here report on a method to adapt microfluidic stickers made from UV-curable optical adhesive with inserted permeable hydrogel membrane micro-windows for mechanical studies of suspended cells. The windows were fabricated by optical projection lithography using scanning confocal microscopy. The device allows us to rapidly exchange embedding medium while observing and probing the cells. We characterize the device and demonstrate the function by exposing cultured fibroblasts to varying osmotic conditions. Cells can be shrunk reversibly under osmotic compression.

Duke Scholars

Published In

Journal of Physics D: Applied Physics

DOI

EISSN

1361-6463

ISSN

0022-3727

Publication Date

January 10, 2018

Volume

51

Issue

4

Related Subject Headings

  • Applied Physics
  • 51 Physical sciences
  • 40 Engineering
  • 09 Engineering
  • 02 Physical Sciences
 

Citation

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Rezvani, S., Shi, N., Squires, T. M., & Schmidt, C. F. (2018). Microfluidic device for chemical and mechanical manipulation of suspended cells. Journal of Physics D: Applied Physics, 51(4). https://doi.org/10.1088/1361-6463/aaa121
Rezvani, S., N. Shi, T. M. Squires, and C. F. Schmidt. “Microfluidic device for chemical and mechanical manipulation of suspended cells.” Journal of Physics D: Applied Physics 51, no. 4 (January 10, 2018). https://doi.org/10.1088/1361-6463/aaa121.
Rezvani S, Shi N, Squires TM, Schmidt CF. Microfluidic device for chemical and mechanical manipulation of suspended cells. Journal of Physics D: Applied Physics. 2018 Jan 10;51(4).
Rezvani, S., et al. “Microfluidic device for chemical and mechanical manipulation of suspended cells.” Journal of Physics D: Applied Physics, vol. 51, no. 4, Jan. 2018. Scopus, doi:10.1088/1361-6463/aaa121.
Rezvani S, Shi N, Squires TM, Schmidt CF. Microfluidic device for chemical and mechanical manipulation of suspended cells. Journal of Physics D: Applied Physics. 2018 Jan 10;51(4).
Journal cover image

Published In

Journal of Physics D: Applied Physics

DOI

EISSN

1361-6463

ISSN

0022-3727

Publication Date

January 10, 2018

Volume

51

Issue

4

Related Subject Headings

  • Applied Physics
  • 51 Physical sciences
  • 40 Engineering
  • 09 Engineering
  • 02 Physical Sciences