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Deciphering Elevated Microsatellite Alterations at Selected Tetra/Pentanucleotide Repeats, Microsatellite Instability, and Loss of Heterozygosity in Colorectal Cancers.

Publication ,  Journal Article
Wang, Y; Vnencak-Jones, CL; Cates, JM; Shi, C
Published in: J Mol Diagn
May 2018

Elevated microsatellite alterations at selected tetranucleotide repeats (EMAST) are common in colorectal cancers (CRCs). The association between EMAST and classic mono/dinucleotide microsatellite instability (MSI) is unknown. We assessed the stability of 13 tetranucleotide and three pentanucleotide repeat markers in tumor and normal tissue from 22 MSI-high and 107 microsatellite-stable CRC samples. When present, instability was observed at tetra/pentanucleotide repeats and was defined as elevated microsatellite alterations at selected tetra/pentanucleotide repeats-high (EMASTP-H; ≥30% instability), -low (EMASTP-L; <30% instability), or -stable (EMASTP-S). EMASTP instability, including high and low, was observed in 50 of 123 CRCs (41%), including all MSI-high tumors and 28 of 101 microsatellite-stable tumors (28%). MSI-high CRCs were more likely to be EMASTP-H compared with microsatellite-stable tumors with EMASTP instability. Tetranucleotide markers VWA and D13S317 were the two most frequently altered loci. Loss of heterozygosity was more common in EMASTP-L/S than in EMASTP-H CRCs. Frequencies of loss of heterozygosity at three loci were different between EMASTP-L and EMASTP-S tumors. In addition, right-sided tumor site, large tumor size, high tumor grade, and the presence of Crohn-like reaction were significantly associated with EMASTP-H CRCs. However, there were no differences in clinicopathologic features between EMASTP-L and EMASTP-S tumors. In summary, more CRCs exhibited genomic instability as EMASTP than as MSI. EMASTP instability may prove to be an important prognostic/therapeutic indicator in CRCs.

Duke Scholars

Published In

J Mol Diagn

DOI

EISSN

1943-7811

Publication Date

May 2018

Volume

20

Issue

3

Start / End Page

366 / 372

Location

United States

Related Subject Headings

  • Pathology
  • Middle Aged
  • Microsatellite Repeats
  • Microsatellite Instability
  • Male
  • Loss of Heterozygosity
  • Humans
  • Female
  • Colorectal Neoplasms
  • Aged, 80 and over
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, Y., Vnencak-Jones, C. L., Cates, J. M., & Shi, C. (2018). Deciphering Elevated Microsatellite Alterations at Selected Tetra/Pentanucleotide Repeats, Microsatellite Instability, and Loss of Heterozygosity in Colorectal Cancers. J Mol Diagn, 20(3), 366–372. https://doi.org/10.1016/j.jmoldx.2018.02.001
Wang, Yang, Cindy L. Vnencak-Jones, Justin M. Cates, and Chanjuan Shi. “Deciphering Elevated Microsatellite Alterations at Selected Tetra/Pentanucleotide Repeats, Microsatellite Instability, and Loss of Heterozygosity in Colorectal Cancers.J Mol Diagn 20, no. 3 (May 2018): 366–72. https://doi.org/10.1016/j.jmoldx.2018.02.001.
Wang, Yang, et al. “Deciphering Elevated Microsatellite Alterations at Selected Tetra/Pentanucleotide Repeats, Microsatellite Instability, and Loss of Heterozygosity in Colorectal Cancers.J Mol Diagn, vol. 20, no. 3, May 2018, pp. 366–72. Pubmed, doi:10.1016/j.jmoldx.2018.02.001.
Journal cover image

Published In

J Mol Diagn

DOI

EISSN

1943-7811

Publication Date

May 2018

Volume

20

Issue

3

Start / End Page

366 / 372

Location

United States

Related Subject Headings

  • Pathology
  • Middle Aged
  • Microsatellite Repeats
  • Microsatellite Instability
  • Male
  • Loss of Heterozygosity
  • Humans
  • Female
  • Colorectal Neoplasms
  • Aged, 80 and over