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Anti-inflammatory effects of naproxen sodium on human osteoarthritis synovial fluid immune cells.

Publication ,  Journal Article
Hsueh, M-F; Bolognesi, MP; Wellman, SS; Kraus, VB
Published in: Osteoarthritis Cartilage
May 2020

OBJECTIVE: To evaluate the anti-inflammatory effects of clinically relevant naproxen sodium (Nx) concentrations on human monocyte-derived macrophages in a controlled in vitro system and human primary synovial fluid (SF) cells. DESIGN: Using phorbol 12-myristate 13-acetate, THP-1 human monocytic cells were differentiated into mature monocyte-derived macrophages in vitro then treated with Nx pre- or post-activating an inflammatory response with lipopolysaccharide (LPS) and hyaluronan (HA) fragments (n = 8/group). Cell culture supernatants were assessed for NF-κB activity and prostaglandin E2 (PGE2), indicating cyclooxygenase enzyme activity. Under Duke IRB approval, primary human SF cells were collected at the time of knee joint replacement (n = 19 individuals) for osteoarthritis (OA), and cultured with LPS, HA and Nx; SF cells were characterized by polychromatic flow cytometry for cell surface markers and intracellular cytokines. RESULT: Compared to placebo treatment of THP-1 cells, low dose Nx (corresponding 27.5-440 mg/L orally) added both pre- and post-activation with LPS/HA, significantly reduced NF-κB activity and PGE2: mean reduction to 73%, 61%, 17% and 10% of placebo, respectively. LPS/HA treatment of primary OA SF cells significantly increased the number of IL-1β producing primary monocytes and macrophages, and by 24 h the overall production of secreted cytokines (IL-1β, IL-6, IL8, and TNF-α). Low dose Nx reduced the percentage of IL-1β producing primary monocytes and macrophages. CONCLUSION: LPS/HA induced inflammation of THP-1 monocytic and primary human SF cells. Low dose Nx both prevented and reduced inflammatory responses of a human monocytic cell line and reduced IL-1β production by primary human SF monocytes and macrophages.

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Published In

Osteoarthritis Cartilage

DOI

EISSN

1522-9653

Publication Date

May 2020

Volume

28

Issue

5

Start / End Page

639 / 645

Location

England

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • THP-1 Cells
  • T-Lymphocytes
  • Synovial Fluid
  • Osteoarthritis, Knee
  • Neutrophils
  • Naproxen
  • NF-kappa B
  • Monocytes
  • Macrophages
 

Citation

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Hsueh, M.-F., Bolognesi, M. P., Wellman, S. S., & Kraus, V. B. (2020). Anti-inflammatory effects of naproxen sodium on human osteoarthritis synovial fluid immune cells. Osteoarthritis Cartilage, 28(5), 639–645. https://doi.org/10.1016/j.joca.2020.01.013
Hsueh, M. -. F., M. P. Bolognesi, S. S. Wellman, and V. B. Kraus. “Anti-inflammatory effects of naproxen sodium on human osteoarthritis synovial fluid immune cells.Osteoarthritis Cartilage 28, no. 5 (May 2020): 639–45. https://doi.org/10.1016/j.joca.2020.01.013.
Hsueh M-F, Bolognesi MP, Wellman SS, Kraus VB. Anti-inflammatory effects of naproxen sodium on human osteoarthritis synovial fluid immune cells. Osteoarthritis Cartilage. 2020 May;28(5):639–45.
Hsueh, M. .. F., et al. “Anti-inflammatory effects of naproxen sodium on human osteoarthritis synovial fluid immune cells.Osteoarthritis Cartilage, vol. 28, no. 5, May 2020, pp. 639–45. Pubmed, doi:10.1016/j.joca.2020.01.013.
Hsueh M-F, Bolognesi MP, Wellman SS, Kraus VB. Anti-inflammatory effects of naproxen sodium on human osteoarthritis synovial fluid immune cells. Osteoarthritis Cartilage. 2020 May;28(5):639–645.
Journal cover image

Published In

Osteoarthritis Cartilage

DOI

EISSN

1522-9653

Publication Date

May 2020

Volume

28

Issue

5

Start / End Page

639 / 645

Location

England

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • THP-1 Cells
  • T-Lymphocytes
  • Synovial Fluid
  • Osteoarthritis, Knee
  • Neutrophils
  • Naproxen
  • NF-kappa B
  • Monocytes
  • Macrophages