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Development of Antibodies with Broad Neutralization Specificities against HIV-1 after Long Term SHIV Infection in Macaques.

Publication ,  Journal Article
Gao, N; Gai, Y; Meng, L; Wang, C; Zhang, X; Wang, W; Qin, C; Yu, X; Gao, F
Published in: Viruses
January 31, 2020

Non-human primates (NHP) are the only animal model suitable to evaluate the protection efficacy of HIV-1 vaccines. It is important to understand how and when neutralizing antibodies (nAbs) with specificities similar to those of human broadly neutralizing antibodies (bnAbs) develop in NHPs. To address these questions, we determined plasma neutralization specificities in two macaques which developed neutralization breadth after long-term simian/human immunodeficiency virus (SHIV) infection and identified neutralization escape mutations by analyzing the env sequences from longitudinal plasma samples. Neutralization activities targeting V2, CD4bs, V3 and gp120-gp41 interface only became detectable in week 350 plasma from macaques G1015R and G1020R using 25710 env mutants. When mapped with CAP45 env mutants, only V2 specificity was detected at week 217 and persisted until week 350 in G1015R. Neutralization escape mutations were found in CD4bs and V2 regions. However, all of them were different from those resistant mutations identified for human bnAbs. These results show that nAbs with specificities similar to human bnAbs are only detectable after long-term SHIV infection and that neutralization escape mutations in macaques are different from those found in HIV-1-infected individuals. These findings can have important implications in the best utilization of the NHP model to evaluate HIV-1 vaccines.

Duke Scholars

Published In

Viruses

DOI

EISSN

1999-4915

Publication Date

January 31, 2020

Volume

12

Issue

2

Location

Switzerland

Related Subject Headings

  • Time Factors
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • Neutralization Tests
  • Mutation
  • Macaca mulatta
  • HIV-1
  • HIV Seropositivity
  • HIV Antibodies
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gao, N., Gai, Y., Meng, L., Wang, C., Zhang, X., Wang, W., … Gao, F. (2020). Development of Antibodies with Broad Neutralization Specificities against HIV-1 after Long Term SHIV Infection in Macaques. Viruses, 12(2). https://doi.org/10.3390/v12020163
Gao, Nan, Yanxin Gai, Lina Meng, Chu Wang, Xin Zhang, Wei Wang, Chuan Qin, Xianghui Yu, and Feng Gao. “Development of Antibodies with Broad Neutralization Specificities against HIV-1 after Long Term SHIV Infection in Macaques.Viruses 12, no. 2 (January 31, 2020). https://doi.org/10.3390/v12020163.
Gao N, Gai Y, Meng L, Wang C, Zhang X, Wang W, et al. Development of Antibodies with Broad Neutralization Specificities against HIV-1 after Long Term SHIV Infection in Macaques. Viruses. 2020 Jan 31;12(2).
Gao, Nan, et al. “Development of Antibodies with Broad Neutralization Specificities against HIV-1 after Long Term SHIV Infection in Macaques.Viruses, vol. 12, no. 2, Jan. 2020. Pubmed, doi:10.3390/v12020163.
Gao N, Gai Y, Meng L, Wang C, Zhang X, Wang W, Qin C, Yu X, Gao F. Development of Antibodies with Broad Neutralization Specificities against HIV-1 after Long Term SHIV Infection in Macaques. Viruses. 2020 Jan 31;12(2).

Published In

Viruses

DOI

EISSN

1999-4915

Publication Date

January 31, 2020

Volume

12

Issue

2

Location

Switzerland

Related Subject Headings

  • Time Factors
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • Neutralization Tests
  • Mutation
  • Macaca mulatta
  • HIV-1
  • HIV Seropositivity
  • HIV Antibodies