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Application of 15N2-Diazirines as a Versatile Platform for Hyperpolarization of Biological Molecules by d-DNP.

Publication ,  Journal Article
Park, H; Zhang, G; Bae, J; Theis, T; Warren, WS; Wang, Q
Published in: Bioconjugate chemistry
March 2020

15N2-Diazirines represent an attractive class of imaging tags for hyperpolarized magnetic resonance imaging (HP-MRI), offering desirable biocompatibility, ease of incorporation into a variety of molecules, and ability to deliver long-lasting polarization. We have recently established hyperpolarization of 15N2-diazirines in organic solvents using SABRE-Shield Enables Alignment Transfer to Heteronuclei (SABRE-SHEATH). Yet, the current challenge of SABRE-SHEATH in water, specifically poor polarization efficiency, presents a barrier in examining the practical use of 15N2-diazirines for HP-MRI. Herein, we show that efficient polarization of diverse 15N2-diazirine-labeled molecules in water can be readily achieved by dissolution dynamic nuclear polarization (d-DNP), a hyperpolarization technique used in clinical practice. Hyperpolarization by d-DNP also demonstrates greater enhancement for long-lasting 15N signals, in comparison with SABRE-SHEATH. Various biologically important molecules are studied in this work, including amino acid, sugar, and drug compounds, demonstrating the great potential of 15N2-diazirines as molecular tags in broad biomedical and clinical applications.

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Published In

Bioconjugate chemistry

DOI

EISSN

1520-4812

ISSN

1043-1802

Publication Date

March 2020

Volume

31

Issue

3

Start / End Page

537 / 541

Related Subject Headings

  • Organic Chemistry
  • Nitrogen Isotopes
  • Magnetic Resonance Imaging
  • Diazomethane
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0305 Organic Chemistry
  • 0304 Medicinal and Biomolecular Chemistry
 

Citation

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Park, H., Zhang, G., Bae, J., Theis, T., Warren, W. S., & Wang, Q. (2020). Application of 15N2-Diazirines as a Versatile Platform for Hyperpolarization of Biological Molecules by d-DNP. Bioconjugate Chemistry, 31(3), 537–541. https://doi.org/10.1021/acs.bioconjchem.0c00028
Park, Hyejin, Guannan Zhang, Junu Bae, Thomas Theis, Warren S. Warren, and Qiu Wang. “Application of 15N2-Diazirines as a Versatile Platform for Hyperpolarization of Biological Molecules by d-DNP.Bioconjugate Chemistry 31, no. 3 (March 2020): 537–41. https://doi.org/10.1021/acs.bioconjchem.0c00028.
Park H, Zhang G, Bae J, Theis T, Warren WS, Wang Q. Application of 15N2-Diazirines as a Versatile Platform for Hyperpolarization of Biological Molecules by d-DNP. Bioconjugate chemistry. 2020 Mar;31(3):537–41.
Park, Hyejin, et al. “Application of 15N2-Diazirines as a Versatile Platform for Hyperpolarization of Biological Molecules by d-DNP.Bioconjugate Chemistry, vol. 31, no. 3, Mar. 2020, pp. 537–41. Epmc, doi:10.1021/acs.bioconjchem.0c00028.
Park H, Zhang G, Bae J, Theis T, Warren WS, Wang Q. Application of 15N2-Diazirines as a Versatile Platform for Hyperpolarization of Biological Molecules by d-DNP. Bioconjugate chemistry. 2020 Mar;31(3):537–541.
Journal cover image

Published In

Bioconjugate chemistry

DOI

EISSN

1520-4812

ISSN

1043-1802

Publication Date

March 2020

Volume

31

Issue

3

Start / End Page

537 / 541

Related Subject Headings

  • Organic Chemistry
  • Nitrogen Isotopes
  • Magnetic Resonance Imaging
  • Diazomethane
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0305 Organic Chemistry
  • 0304 Medicinal and Biomolecular Chemistry